| China whose shrimp aquaculture production had reached 31% of the national total is a major producer of breeding shrimp, However, due to the development of intensive aquaculture, the blind expansion of farming and farming scale over-exploitation of water and damaged the ecological balance of water, causing the large population of pathogens to the aquaculture industry in China has brought huge economic losses. According to statistics, only the outbreak of the virus in 1993 made a loss of 80 billion RMB, So how to take effective measures to control these diseases has become a top priority. At first people use drugs to prevent and treat microbial infection in shrimp. However, the generation of drug-resistant microbes brought a new problem to aquaculture, and shrimp disease in the aquaculture industry frequently suffered major losses. Also the drug residues in shrimp harm to human body and pollute the environment. Therefore, Polysaccharide becomes a research hotspot in recent years. The yeast cell wall polysaccharides have been shown to be a good immune enhancers, it can speed up the shrimp immune organ development, enhance and stimulate non-specific. However, the yeast cell wall polysaccharides in the application of aquaculture will be affected by many factors. Therefore, the application, suitable amount and processing time of the yeast cell wall polysaccharides become the current research focus. In this study, the following four groups to probe into the yeast cell wall polysaccharides on the immune system of M. rosenbergii, for such substances to the immune system of M. rosenbergii provide the basis for the activation mechanism.1,The effects of some immune related isozymes when M. rosenbergii is soaked in different concentration yeast cell-wall polysaccharidesThis research first made M. rosenbergii soked in 0.00 mg/l, 31.25 mg/l, 62.50 mg/l, 125.00 mg/l, 250.00 mg/l five concentration yeast cell-wall polysaccharides for 120h, and then measure the enzyme activity and combine electrophoresis to explore the effects of some immune related isozymes(acidic phosphatase (ACP), peroxidase (POD), phenolic oxidase (PPO) and superoxide dismutase (SOD)) from heart, liver, gill and muscles. The changes of four isozymes were analyzed in different tissues of M. rosenbergii. The results showed that four isozymes of expression containe clear tissue specificity. Along with the increase of the concentration of the yeast cell-wall polysaccharides, zymography of gill has a most obvious change, followed by liver and heart tissue, muscle tissue zymography hasn't significant change. The enzyme activity also showed the same results. And the zymograms and activities of four isozymes are all changed obviously when the yeast cell-wall polysaccharides concentration is 125.00 mg/l. Therefore, soaking M. rosenbergii in 125 mg/L concentration yeast cell-wall polysaccharides could strengthen the bodys immunity,2,The effects of some immune related isozymes when M. rosenbergii have been soaked in yeast cell-wall polysaccharides solution for different timeIn this study, M. rosenbergii was soaked in yeast cell-wall polysaccharides solution, and then measure the enzyme activity and combine electrophoresis to explore the effects of some immune related isozymes(acidic phosphatase (ACP), peroxidase (POD), phenolic oxidase (PPO) and superoxide dismutase (SOD)) from heart, liver, gill and muscles every 48h. The result showed that the activity of ACP in all tissues, POD and PPO in heart and gill, and SOD in heart increased significantly after M. rosenbergii was soaked in yeast cell-wall polysaccharides solution for 144h. But ACP and PPO activity have a big decreased after 192h. The physiological mechanism of the phenomenon may be related to living organism negative feedback regulation system. So soaking M. rosenbergii yeast cell-wall polysaccharides for 144h could strengthen the bodys immunity.3,The effects of some immune related isozymes when M. rosenbergii is injected with different concentration yeast cell-wall polysaccharidesThis research first inject M. rosenbergii with 0.1ml of 0.0%,1.0%,2.0%or3.0% yeast cell-wall polysaccharides solution every shrimp for 72h, and then measure the enzyme activity and combine electrophoresis to explore the effects of some immune related isozymes(acidic phosphatase (ACP), peroxidase (POD), phenolic oxidase (PPO) and superoxide dismutase (SOD)) from heart, liver, gill and muscles. The result showed that the zymograms and activities of four isozymes changed obviously when M. rosenbergii is injected in 2.0% concentration yeast cell-wall polysaccharides. The isozymes in muscle changed strongest of all, because the activities of ACP,PPO and SOD reached the peak , respectively, 38.995 U/L,22.517 Eu/(g.min)and 12.297 U/mg. The ACP activity of heart and POD activity of gill also increase obviously. So injecting M. rosenbergii 2.0% concentration yeast cell-wall polysaccharides could strengthen the bodys immunity4,The effects of ACP and POD when M. rosenbergii have been injected with yeast cell-wall polysaccharides solution for different timeIn this study, M. rosenbergii was injected with yeast cell-wall polysaccharides solution, and then measure the enzyme activity and combine electrophoresis to explore the effects of ACP and POD from heart, liver and muscles after 24h,48h,72h,96h和120h. ACP activity in the heart and muscle were significantly different with the control group, respectively reached the peak of 93.0U/L and 34.5 U/L after 72h. But ACP activity in the liver reached the maximum 284U / L after 96 hours. The ACP activity in these three kinds of tissues were maintained high level, even until after 120h ACP activity were still significantly higher than the control group. The POD activity assay data showed that POD activity in liver and heart respectively increased to the peak of 5.38 Eu/(g.min)and 23.16 Eu/(g.min)after 72h or 48h. And the high level of POD activities in these two tissues have maintained for only 24h, and then respectively down to the control group after 96h and 72h. The POD activity in muscle has improved but is still not very satisfactory. In short, the experiment results showed that just after 72h M. rosenbergii injected with yeast cell-wall polysaccharides solution has a great improvement in immunity.In summary, soaking and injection methods can induce humoral immune responses of M. rosenbergii to improve a variety of immune activity, thus enhancing disease prevention and disease resistance. And this study also confirmed that soaking M. rosenbergii in 125.00mg/l of yeast cell wall polysaccharides for 144 or injecting 2% of the yeast cell wall polysaccharides can strengthen the shrimps immunity. However, in the production practice, the method of jection is time consuming and difficult operation, and can not apply to individual small shrimp. The method of soaking will cost larger dosage, but only a little absorbed by shrimp, so further study on oral is necessary. In the experimental operation, a comprehensive analysis of all factors is needful for determining the most appropriate method.
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