Effects Of Yeast Cell Wall Polysaccharide On Performance And Immune Of Broilers

The experiment was conducted to investigate the effect of dietary yeast cell wall polysaccharides performance and immune function of broilers fed with normal corn diet or dietary aflatoxin B1 or Escherichia coli infection three cases.Experiment 1 using single factor design,1200 poultry according to the weight evenly distributed to five processing, each processing 6 repeat, each repeat 40;experiment 1 the whole period in addition to processing 1 feeding based diet in comparison the rest processing respectively fed different experiment 1 diet (basal diet +cell walls of baker’s yeast polysaccharide 1000 g/t. beer yeast cell wall polysaccharides of 1000 g/t,500 g/t activate the yeast cell wall polysaccharides, activation of yeast cell wall polysaccharide 1000 g/t);Record the production performance and the age of 21 and 42 days of age is sampled blood and slaughter, determination of immune organ index, innocent blood globulin ratio, cell factor and Newcastle disease antibody titer. The results show that under the condition of normal feeding,1000 g/t bread yeast cell wall polysaccharides,500 g/t activate the yeast cell wall polysaccharides and 1000 g/t activate the yeast cell wall polysaccharides had a tendency to reduce the chicken later selective,500 g/t activate the yeast cell wall polysaccharides has a tendency to decrease the rate of chicken died late tao;Body weight gain in 1000 g/t of beer yeast cell wall polysaccharides have reduce late and full period of the trend of body weight;And 1000 g/t of beer yeast cell wall sugar significantly decreased and full period of the late feed intake, so the material weight ratio did not change significantly;The source of yeast cell wall polysaccharides of chicken immune organ index had no significant influence; 1000 g/t bread yeast cell wall polysaccharides,500 g/t activate the yeast cell wall polysaccharides and 1000 g/t activate the yeast cell wall polysaccharides has a tendency to reduce the chicken blood white balls than 21 d;Cytokines, the early stage of the 1000 g/t bread yeast cell wall polysaccharides increased; the level of IL-2Newcastle disease antibody titer,1000 g/t of beer yeast cell wall polysaccharides group in the early stage of the rising trend.Experiment 2 use 2×4 factorial design, mouldy maize 0%,0% two levels, the yeast cell wall polysaccharide including 0,1000 g,400 g/t/t, and yeast cell wall and 100 g/400 g/t t selenium combination of four levels, a total of eight processing, each processing 6 repeat, every repeat only a total of 960 only 20 chickens;Test the feeding experiment diet;Record the production performance and in 21 and 42 days of age blood and slaughter sampling, determination of immune organ index, innocent blood globulin ratio and serum antioxidant.Results show that the mouldy corn poultry deaths, selective and body weight were not significantly influence, but a significant increase in the late broilers (P= 0.027) and the phase of feed intake (P= 0.014), the late broilers and full-term heavier than significantly increased (P< 0.001), the effect is improved after adding yeast cell wall polysaccharides.Mildew corn of yeast cell wall polysaccharides of poultry immune index were not significant impact, but there are 42 d’s French bursa index between the two interaction effect (P= 0.046);Mildew corn in 42 d significantly increased (P= 0.007) in serum total protein and albumin levels (P= 0.006), while the white ball than without significant effect, at the same time, mould and yeast cell wall polysaccharides corn (P= 0.018) in the early and late (P= 0.034) white ball than to have interaction effect;Mildew corn significantly reduce early serum total antioxidant capacity (P= 0.004), and yeast cell wall polysaccharides in the later significantly increased serum total antioxidant capacity (P= 0.001), and the interaction with the mildew corn (P= 0.021);Mildew corn in the prophase of serum glutathione peroxidase were not significantly affect, the yeast cell wall polysaccharides in prophase significantly reduced glutathione peroxidase activity in serum (P< 0.001), and the interaction with the mildew corn (P< 0.001), mildew corn in the later significantly increased glutathione peroxidase activity (P= 0.007), while the yeast cell wall polysaccharides significantly drop trough the vitality of glutathione peroxidase (P< 0.001), and both have interaction effect (P= 0.012):In addition, mould and yeast cell wall polysaccharide corn additive of peroxidase (pod) activity in serum were not significantly affected, but both have interaction effect (P= 0.004), while the late mildew corn (P= 0.035), and yeast cell wall polysaccharides (P< 0.001) were significantly lower serum catalase vigor, and both have interaction (P= 0.002).Experiment 3 use 2×3 design, including e.coli into poison poison attack and don’t attack two levels, the yeast cell wall polysaccharide including 0,1000 g,2000 g/t/t three levels, a total of six processing, each 6 repeat, repeat each repeat four only a total of 144 chickens.Experiment all phase feeding diet, in the age of 21, using 02 serotype pathogenic escherichia coli oral lavage against poison, poison attack dose for 1 ml2.1*108 cfu/mL bacteria liquid, not poison attack group use 1 mL sterilization LB culture medium to fill the stomach;After tapping poison to continue feeding experiment diet:Before the attack poison, poison attack as 2 d.7 d.14 d after repeated each choose a chicken blood and slaughter sampling, intestinal development index and serum cytokines.Attack the results showed that e. coli decreased significantly after the poison of the duodenum, jejunum, ileum villus length, higher crypt depth, to reduce the pile than Cain (P< 0.001), the yeast cell wall polysaccharides can not only improve influence on gut, e.coli to return to normal levels, intestinal development and is also a significant boost when in uninfected escherichia coli than Cain, decrease in the length of the intestine villi crypt depth (P< 0.001) effect, and this effect is dose-dependent effect;Marked increase in the e. coli infection after 2 d the levels of serum IL-6 (P< 0.001), at the same time, the yeast cell wall polysaccharide at the same time also significantly raised the level of IL-6 (P< 0.001), and there is interaction effect (P= 0.003);Poison attack after 7 d e. coli was significantly lower levels of serum IL-1 (P= 0.002), and interactions with the yeast cell wall polysaccharides (P= 0.011);The marked increase in the e. coli infection after 14 d serum IL-1 level (P-0.009), and yeast cell wall polysaccharide has the inhibition effect on the level of IL-1 at this time, but not significant.These results indicate that the yeast cell wall polysaccharides can raise chicken in chicken by immunosuppressive feed conversion rate, regulate the body’s immune function, and affected by the e. coli infection in intestinal development to protect poultry intestinal from the damage of e. coli, guarantee the chicken to the absorption of nutrition.