| In this study, in order to research heterotic and genetic distance of inbred lines exoticed Canada and important lines representing different heterotic groups in Heilongjiang province, we used combining ability analysis combined with SSR molecule marker clutering analysis. The result will greatly influence to make full use of indroduced lines.Through analysis of combining ability, heterotic, genetic distance, heterozqosity and their correlations with F1 grain yield, discuss the feasibility and effect of heterotic prediction, Following are the main conclusion of the paper:1. 120 combinations were designed according to North Carolina mating design II (NC II ), the result of combining ability analysed shown that GCA of grain weight lines introduced Canada changed from -13.0104～26.5603, GCA of C2, C4, C6, C12, C14, C15 were positive, and among them GCA of C6 and C14 were maximum, 15.7572 and 26.5603 respectively. GCA of grain weight of the C14×B14 and the C14×H lv 28 is remarkable positive number, each was 11.1264 , 16.7791 and 38.5218, so C6 and C14 had great value in direct utilization, and can hybridized with excellent heterotic group to choose strong heterotic. GCA of C1, C3, C5, C7, C8, C9, C10, C11, C13 were negative, and among them GCA of C10 and C11 were least,-13.0104 and -10.0621 respectively, so they had low production if hybridized with heterotic group. Through GCA analysis of ear perimeter, grain longest, rows per ear, kernel per row, hundred grain weight, the result shown that GCA of ear perimeter, grain longest, rows per ear, kernel per row, hundred grain weight were C14(23.9557), C6(8.1012), C13(7.6932), C14(11.6369)and C7(11.7085)respectively. These lines can be used for improve character of low heterotic group.2. Clutering analysis according to total combining ability of plot yield method, the result shown that 15 inbred lines introduced Canada classified into 4 clusters, the first clusters including C1, C2, C3, C4, C8 and C11, the second clusters including C5 and C10, the third clusters including C6, C7, C12, C13, C14 and C15, C9 clusters alonely. Future analysed the relation of 4 clusters and experiment inbred lines, the relation of the first clusters and dan340 inbred lines was close, the relation of the second clusters and K10 inbred lines was close, the relation of the third clusters and inbred lines was far, the relation of C9 and dan340, H lv28, K10, W557 was close.The experiment selected of 67 paises SSR primers, including 50 paises polymorphic primers. We detected 214 polymorphic bands among 23 inbred lines, related to 50 pairs primers. There are 3.4 alleles on each primer loci, change range from 2 to 9 and primer phi126 was highest. Average polymorphisminformation content (PIC) was 0.613, change range from 0.22~0.85, primer nc133 was lowest and primer bnlg161 was highest.3. A cluster analysis using SSR data classified the 15 inbred lines introduced Canada and 14 important lines representing different heterotic groups in our country into 5 clusters, the first clusters including C1, C2, C5, C7, C3, C6, C9, C4, C8, C12, C11, C10 and H lv28, the second clusters including C13, C15, C14 and B14, the third clusters including dong46, 446, longkang11, Mo17, the fouth clusters including jing7, dan340, W557 and K10, the fifth clusters including he344, za1, za c546, hai074. The two cluster methods can pull C1, C2, C3, C4, C8 and C11 into Luda Red Cob Pedigree. Clutering analysis according to total combining ability of plot yield and SSR molecule marker clutering analysis of inbred lines exoticed Canada was different.4. The result shown that only through molecule marker genetic distance could not be predicted heterotic correctly. According to SCA of production character can predicted heterotic accurately.
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