Effects Of Slope, Slope Positions And Slopes On Part Of Enzyme Activity In Pinus Koraiensis Needles

Korean pine (Pinus koraiensis) is the second-class national protected plants. It is one of the main forest tree species in northeastren China which one's economic value and ecological value are both precious.Based on the Korean pine needles as the main research object, using the orthogonal experiment site factors on the explorative to study Korean pine needles under natural conditions in chemical defense substance-protective enzyme, defense enzymes and protease inhibitors, explore the influence of active chemical defense physical activity within the needles on the regularity and natural habitats of physiological adaptations of different habitats mechanism to the appropriate management under Korean pine forest to provide theoretical basis. The experimental results are as follows:1. The influence of protective enzyme activity in Korean pine needles under the Site factors:The protection enzyme activity in Korean pine needles are both significant correlation with slope (from 3°to 25°), the activity of superoxide dismutase (SOD) and peroxidase (POD) were positively correlated with slope, the activity of catalase (CAT) was negatively correlated with slope. The protection enzyme activity in Korean pine needles are no significant effect with slope positions.The progression for SOD activity of Korean pine needles under the site factors is slopes, slope and slope positions. The progression for POD activity of Korean pine needles under the site factors is slope, slopes and slope positions. The progression for CAT activity of Korean pine needles under the site factors is slope, slope positions and slopes.The protection enzyme activity in different direction of Crown has significant difference, the maximum activity of SOD and POD occurs in west crown, and the maximum activity of CAT occurs in the south.Research found that the activity of SOD and POD Change consistent, and the activity of CAT Changes instead.2. The influence of defense enzyme activity in Korean pine needles under the site factors:The activity of phenylalanine solution ammonia enzyme (PAL) in Korean pine needles was significant correlation with slope, and the activity of polyphenol oxidase (PPO) was positively correlated with slopes.The progression for PAL activity of Korean pine needles under the site factors is slope, slopes and slope positions. The progression for PPO activity of Korean pine needles under the site factors is slopes, slope and slope positions.The defense enzyme activity in different direction of Crown has significant difference, the maximum activity of PAL and PPO occurs in the north.3. The influence of protease inhibitors in Korean pine needles under the site factors:The activity of protease inhibitors has no significant correlation with the site factors.The progression for TI activity of Korean pine needles under the site factors is slope, slope positions and slopes. The progression for CI activity of Korean pine needles under the site factors is slope, slope positions and slopes.The protease inhibitors activity in different direction of Crown has no significant difference.This study selected samples with orthogonality scientifically and reasonably, optimized the experiment scheme, reduced errors repeated. Through research the chemical defense material activity of Korean pine needles under different orientation and site conditions, the result is that the protective and defense enzyme of the natural growth Korean pine in different site conditions had differences, and the site factors on the activity of protease inhibitors was ignore. We will continue to careful investigation and study, provide sufficient theoretical basis for Foster resistant Korean pine tree species by selecting the optimal site conditions.