| In this study, the lea/bZIP divalent gene, which were cloned from extremely drought and salt-tolerant Tamarix plant, was transformed into tobacco, then the drought and salt-stress test of transgenic tobacco and non-transgenic line was performed. The results are shown as follows:Eight kannamycin resistant lines were selected for analysis. PCR detection showed all of them were positive. The result proved the lea /bZIP divalent gene has been integrated into the tobacco genome. Northern blotting hybridization shows overexpression of lea/bZIP in transgenic tobacco.Three transgenic lines (T1) and control were tested under tissue culture condition. The result of salt stress experiment showed:in 200mmol/L NaCl concentration, the injury of transgenic tobacco leaves is less than non-transgenic tobacco, the height transgenic tobacco were higher 33.8% than control, the seed germination of transgenic tobacco was 58.0% higher than control.Three transgenic lines (T1)and control were tested under soil condition. The result of salt stress experiment showed: after 7 day deal with 200mmol/L NaCl concentration, the average MDA content of transgenic tobacco 20.8% lower than control. The average POD activity of transgenic tobacco slightly higher than control, the soluble protein content of transgenic tobacco and control was increased at frist, then decreased. The average chlorophy II content of transgenic tobacco 21.0% higher than control.The germination rate of seed was tested on three transgenic lines (T1) and control. The result of drought stress experiment showed:under the culture with 8% PEG MS medium, the seed germination rate and germination conditions were better than control. Under 200mmol/L mannitol treatment after 7days, the average height of transgenic tobacco 10.16% higher than control.All in all, the expression of lea/bZIP divalent gene could enhance the resistance of the salt and drought stress. |
PDF Full Text Request