Whole Genome Identification Of Tobacco BZIP And Expression Analysis Of A Subfamily Under Stress Treatment

Tobacco is China’s main economic crop.However,in the production process,tobacco planting and production are often suffered hugely due to harsh environment due to geographical constraints and climate change.Abiotic stress factors such as drought,low temperature and high salt are one of the main factors affecting tobacco production.bZIP transcription factor is widely involved in plant growth and development and regulation of stress resistance.It belongs to an important family of transcription factors,and studies tobacco from a whole genetic perspective.The molecular genetic mechanism of abiotic stress in this family will provide theoretical support for subsequent stress-resistant breeding.In this study,the genome-wide identification and bioinformatics analysis of the bZIP gene family related to tobacco abiotic stress response,and the expression analysis of 18 genes in the Group A under the four abiotic stress treatments The structure and function of the bZIP gene family were initially predicted and studied.The main results are as follows:A total of 105 genes containing the bZIP conserved domain were identified in the tobacco whole gene using Hmmer software based on Hidden Markov Model and pfam’s online protein structure.The protein sequence length was 112 to 973 and the molecular weight distribution was 12913.63.-104640.38 Da,the conserved domain sequence length is 35-59.Its isoelectric point is distributed between 4.91 and 9.79,and 105 protein sequences are all hydrophilic proteins.A phylogenetic tree was constructed for all the obtained tobacco bzip family sequences,and the 75 sequences of Arabidopsis thaliana were used as controls to divide them into 10 subfamilies,namely A-I and S.The phylogenetic tree of Arabidopsis thaliana and tobacco bZIP transcription factors reveals its evolutionary process.The MEME online software predicted that there are 20 conserved sequences in the tobacco bZIP gene family,and one of the conserved sequences is ubiquitous in103 genes,and the types and numbers of conserved sequences in each subfamily are similar,indicating the grouping.Reliability.Through the stress treatment of common tobacco,including NaCl treatment,PEG6000 treatment,ABA treatment and low temperature(4 °C)treatment,the expression patterns of 18 genes in the A ubfamily of tobacco bZIP gene family were analyzed by q RT-PCR.After analysis,after Na Cl treatment,PEG treatment and ABA treatment,18 genes were up-regulated by stress and up-regulated at different time points.After low-temperature treatment,18 genes were down-regulated by stress,and with The time change gradually decreased and reached the lowest at 24 h,indicating that Na Cl treatment,PEG treatment and ABA treatment induced 18 gene expression,while low temperature treatment inhibited 18 gene expression.It is speculated that the gene of A subfamily participates in the adverse effects of tobacco against abiotic stress,which can provide a reference for further application of genetic engineering and genetic analysis to verify the function of tobacco bZIP gene family.