| Silkworm moth has a very considerable use value, which is rich in protein, fatty acids, amino acid and other ingredients. It is applied in many industries, such as food, pharmaceutical, agricultural, chemical, etc. In a word, it is an rich and precious resource. In the biological field, the female moth egg of silkworm, this is conducted as intermediate hosts in breeding parasitoids Trichogramma, is used widely; while the caesarean section of the silkworm after being ovulated was discarded. In this paper, we took female moths residue that is collected during the industrial production of Trichogramma as raw material. Through experiments, we determined the optimum method of the oil extraction; then, using GC-MS, we analyzed the fatty acid composition of female moth oil by methyl, and studied eight different varieties of Tussah pupae,during its growth and development, in dry weight, moth oil production, and fatty acid changes; those all provided a favorable precondition for the development and utilization of silkworm moth. And they offered a theoretical basis to make use of the antheraea pernyi residue during the process of Trichogramma.1. This paper analyzes the three kinds of moth reported methods, such as soxhlet extraction, extraction, and ultrasonic extraction. Then, we study on the influences to liquid ratio, which are relate to the parameters of the extraction temperature, extraction time, and ultrasonic power. The results show the method of Soxhlet was the best extraction (petroleum ether as solvent,60 to 90, liquid ratio 1:10, temperature is 70℃, extraction time is 6 hours), and the maximum extraction rate can be achieved 20.54%. And with this method of oil extraction, the Oil from antheraea silkworm moth, is good not only has a good color, but also has a light. smell.2. By gas chromatography-mass spectrometry method, we identified and analyzed the components of the female silkworm moth oil residue and its fatty acids. Trough GC-MS, we knew there 10 kinds of fatty oil; and unsaturated fatty acids accounted for 68.43%, among of them, linolenic acid and linoleic acid accounted for 57.17%,10.18% respectively. The unsaturated fatty acid content of the silkworm moth residues is up to 65.77%, of which the content of linolenic acid as high as 55.00%.3. Linolenic acid of tussah oil fatty acids were separated and identified by the method of silica gel column chromatography. We took petroleum as the eluent, through the process of loading, elution, TLC, and then combined; and ultimately we separated linolenic acid and linoleic acid from the unsaturated fatty acids, which purity could be up to 95%.4. We studied eight different varieties of silkworm pupae and determined the changes of oil content under the different accumulated dry weight. The results showed that the dry weight of silkworm pupae decreased with the increase of the accumulated temperature. And its dry weight reduced to a minimum after the silkworm moth emergence; Tussah oil production rate also decreased at the same time.5. Making use of the weather, we quantitatively determined the composition of linolenic acid and linoleic acid of silkworm moth oil. Through the comparison of the fatty acids between eight different varieties of silkworm moth, we found that the content of linoleic acid and linolenic acid of number five is higher than other varieties.
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