Investigation Of Disease Of Artificial Breeding Haliotis Discus Hannai Ino In Fuzhou Region

Haliotis discus hannai Ino is a member of a large class (Gastropoda) of molluscs having one-piece shell which is one of the most commercially important cultured species in China for the edible and medical value. In the 70 years, Haliotis discus hannai Ino was introduced to Fujian Province and since 90 years it has become the primary abalone species in the coast of Fuzhou region. But now the massive death causing by various abalone diseases is a ubiquitous problem. To find out the causes of diseases and raise an effective way to deal with the problem, we investigated the breeding condition and environs of important cultural area in Fuzhou region. Revelant methods of bacteriology was employed to identify the potential pathogenic bacteria from the sick or death abalones and infection test were done to affirm the pathogenic bacteria.1. The condition of several main breeding area (Luoyuan Gulf, the sea area of Fuqing and Pingtan) were investigated, and the data of chemical indexes in breeding area detected by the relative monitoring agency were analyzed. The results suggested that the sea water quality in area of the abalone cultivation achieved the standard"GB 3097-1997"and met the fishery product breeding request. But in the breeding process, people only chased the yield of abalone and ignored the importance of scientific cultivation. Moreover they despised the choice of the varities. Although some varities are cheap, but they can not efficiently resist high temperature and diseases.2. 14 bacteria isotates were isolated and purified from sick or death abalones. After gram staining and observing the shape through microscope, we found that they were all gram negative isotates and helicobacters which only had some small differences of the size and curvature. Biochemical assay and VITEK-32 automatic microorganism identification system were employed to identify the isotates. The results showed that these isotates were all vibrios, including Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio fluvialis-II. To investigate the pathogenicity of these bacteria, 4 typical strains of bacteria were used to infect the healthy abalone by bathed infection, muscle trauma infection and repeated infection respectively. The results showed that all the tested microorganisms are pathogenic to the abalone.