Effect Of Fasting On MSTN Expression In Large Yellow Croaker (Larimichthys Crocea)

Myostatin, originally termed growth differentiation factor-8 (GDF-8), is a member of the transforming growth factor-β(TGF-β) superfamily, has been established as a potent negative regulator of skeletal muscle growth and development in several vertebrates. MSTN was first characterized in the mouse and the individual muscles of the MSTN null mice were 2-3 times heavier than wild-type littermates. MSTN has a potential role in the improvement of animal production. It is shows that if inhibit the activity of MSTN,we can enhance muscle content of cultured species, promoting the development of cultured species.As a result of genome duplication, MSTN is present in double copy. MSTN is generally expressed in skeletal muscles of mammals. In fish, however, its function may be more diverse and may influence many other tissues as it's widely express. This study provides evidence of differentiation of MSTN paralogs by comparatively exploring their tissue-regulation in the large yellow croaker (Larimichthys crocea) when subjected to fasting stress.Results showed differential regulation as well as specific tissue-responses in the muscle, liver, gill, brain, spleen, eye, intestine and kidney of Larimichthys crocea after nutritional deprivation. In particular, the LcMSTN-1 expression increased in liver, muscle, eye and intestine; diminished in gill; remained stable in brain and kidney; in spleen, the LcMSTN-1 expression diminished at first and t increases later. LcMSTN-2 remained stable in eye and muscle; up regulated in spleen and kidney; diminished in intestine, gill and brain; in liver, the LcMSTN-2 expression increases at first and diminished later. Differential tissue-regulation of MSTN paralogs induced functional differentiation in different tissue of Larimichthys crocea. LcMSTN-1 plays an important role as negative regulator of skeletal muscle growth in fish while LcMSTN-2 may be related to the development of brain. Additionally, it is possible that MSTN paralogs involving, directly or indirectly with osmoregulation and non-specific immune and should be target of future investigations.