Genetic Diversity Of Several Fish Populations In Dongping Lake Using Microsatellite Markers

Twenty-seven (Twenty-six) pairs of microsatellite primers developed from common carp were applied to scan genomic DNA of Cyprinus carpio L., Carassius auratus , Pseudobagrus fulvidraco, and Pseudorasbora parva, respectively, in Dongping Lake, Shandong Province, and obtained several pairs of microsatellite primers which were applicable in these fish breeds above. Furthermore, each fish breed genetic structure and diversity were analyzed using those microsatellite loci, the results were described as follows:1. Cyprinus carpio L.: 13 microsatellite loci were polymorphic in all three populations, the genetic diversity level of three Cyprinus carpio L. populations was high with SY population was the highest, WS population was lower,and DP population was the lowest.In order to check the depature of microsatellite loci from HWE,the genotypic frequencies was calculated. Furthermore, 32 cases of observed heterozygosity excess(Fis<0) were observed in three populations. The genetic similarity index between WS population and SY population was the highest(0.8320) among three populations, and their genetic distance was the lowest(0.1680), which indicated that the phylogenetic relationship between these two populations was quite near. On the other hand, the genetic similarity index between WS population and DP population was the lowest(0.8288), and their genetic distance was the highest(0.1712), which indicated that the phylogenetic relationship between these two populations was quite far.2. Carassius auratus: 6 microsatellite loci were polymorphic in both populations, a total of 38 alleles were present among 8 loci, yielding 1 to 6 alleles per locus. The average observed and expected heterozygosity ranged from 0.1471 to 0.9697, and from 0.1422 to 0.7989, respectively. None of the observed genotype frequencies at each locus were in agreement with...