Pharmacokinetics Of Rhubarb In Grass Carp

Objective: High Performance Liquid Chromatography method wasestablished to study the pharmacokinetics of Rhubarb in healthy grass carp after oral administration. Methods:36 healthy grass carps were randomly divided into three groups and given Rhubarb decoction at the dose of 3, 5, 7g/kg · b · w by oral administration, respectively. The plasma concentrations of rhein and emodin were quantified by High Performance Liquid Chromatography (HPLC) assay. The pharmacokinetic parameters were calculated by 3p97 PK program. Results: The absorption, distribution and elimination of rhein and emodin in plasma were both fit one-compartment model with 1st order absorption (weight=1/C2). The main pharmacokinetic parameters and calculated- equations in grass carps were as follows: Rhein of Low dose group: Ke=0. 137±0. 0493h^-1, Ka=1. 262±0. 4713h^-1, T(peak)=2. 102±0. 3849h, C_max= 1. 638 ± 0. 2660mg/L, AUC=17.32 2 ± 4. 3309mg/L ·h, t_1.2(Ke)=5. 783 ±2. 448h, C=2. 59(e^-0.14t-e^-1.26t) ; Middle dose group: Ke=0. 188 ± 0. 0387h^-1, Ka=0.947±0. 2568h^-1, T(peak)=2. 193±0. 2674h, C_max=3. 444± 0. 4221mg/L, AUC=28. 761 ±6. 3892mg/L · h, t_{1 2}(Ke)=3. 844 ± 0. 8269h, C=6. 86(e^-0.19t-e^-0.95t ;High dose group: Ke=0. 150 ± 0. 0184h^-1, Ka=0. 860± 0. 1814h^-1, T(peak) =2. 516 ±0. 3434h, C_max=4. 937 ± 0. 452mg/L, AUC=45. 520±12.1288mg/L · h, t_{1 2}(Ke)=4. 706 ± 0. 6795h, C=8. 87(e^-0.15t-e^-0.86t). Emodin of Low dose group: Ke=0. 154 ± 0. 0216h^-1, Ka = 1. 433 ± 0. 1924h^-1, T(peak)=1. 758 ± 0. 1168h, C,=1. 230±0. 0965mg/L, AUC=10. 585 ± 1. 1578mg/L · h, t_1/2(Ke)=4. 566 ± 0. 5656h, C=1.83(e^-0.15t-e^-1.43t) ;Middle dose group: Ke=0. 166 ± 0. 0170h^-1, Ka=1.252±0. 2327h^-1, T(peak)=1. 899 ± 0. 272h, C_max=2. 106 ± 0. 2363mg/L,AUC=17. 514 ±2. 1498mg/L · h, t_1/2(Ke)=4. 226 ± 0. 4247h, C=3. 37(e^-0.17t-e^-1.25t );High dose group:Ke=0. 160 ± 0. 0264h^-1, Ka=1.198 ±0.2711h^-1, T(peak) =1.989 ±0. 2719h, C_max=3. 985 ± 0. 3497mg/L, AUC=34. 835 ± 3. 3479mg/L · h, t_1/2(Ke)=4. 444 ±...