| Seedless grape is one of important direction of consumption and breeding. However, the most dominating cultivars are Vitis vinifera and sensitive to disease. The Chinese wild Vitis are important gene resources which are highly resistant to fungal disease of grapes. It is an effective approach to breed the resistance cultivars of seedless grape by transferring resistant gene into the cultivars of Vitis vinifera. The full length sequence of novel Glyoxal Oxidase related gene (GLOXrg) from the Chinese Wild Vitis pseudoreticulata Baihe-35-1 was cloned by mRNA RACE in our research team. In this study, we constructed the plant expression vector of GLOXrg, and then we introduced the aim gene into the seedless cultivars of Vitis vinifera (Thompson Seedless, Delight Seedless and Omlo Seedless) and the model plant tobacco. The results were as follow:1. Obtained the germfree tuble seeding of the seedless grapeThe grapevine dormancy branches were burying in the wet sand under low temperature, 4℃. Then the buds were smeared with the 10% calcium cyanamide, inserted and germinated. The sterile system was set up with the germinating shoots. The colony was proliferated with simple bud after the first culture. The fitting subculture medium was NN69+ 0.1 mg/L IBA and the fitting rooting medium was 1/2MS+0.1 mg/L NAA.2. The regeneration system of organs were set upThe grape's leaves, petioles and stem were transferred the different regeneration culture and researched the regeneration. The selected results indicated that the fitting regeneration medium of leaf was MS+2.0 mg/L TDZ+0.1 mg/L NAA+500 mg/L CH+5 g/L PVP and the fitting regeneration medium of petiole and stem was NN69+4.0 mg/L TDZ+0.1 mg/L NAA+500 mg/L CH+5 g/L PVP.3. Regeneration of the embryoid... |
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