Studies On The Absorption And Utilization Of Amino Acids By The Intestines Of C.auratus And Grass Carp In Vitro

Protein plays an important role in the animal nutrition. In essence, the nutrient functions of protein are amino acids,.Protein needs to be digested into free amino acids or small peptides and then be absorbed into animal intestines.The uptake takes on a conjunct task in the course of the whole protein metabolism in animal body.So ,the research concerning amino acid absorption by intestines has been always a hot point put great attention to by animal nutrition experts.At present,the absorption mechanism of amino acids through intestines has been reported largely about mouse,rat,rabbit,domesticanimal,poultry and humanbeings,but few about aquatic animals. Expecially,the reports about amino acids absorbed for synthesizing protein,fat and the other materials in small intestines are very few to be seen.Contents and Purpose:The studies preliminarily focused on the absorption,transportation and utilization of L-Tyrosine and L-Proline by the intestines of C.auratus and Grass carp in Vitro,and on this basis,the research on the absorption and transportation of amino acid from the four hydrolyzed protein feeds by enzyme respectively had been made.we hoped that we could supply some useful basic datas and find a brief and effective experimental method. Text One: Studies on the Absorption and Utilization of L-Tyrosine and L-Proline by the intestines of C.auratus and Grass carp in VitroStudies were conducted to determine the absorption and utilization of L-Tyrosine and L-Proline by the intestines of C.auratus and Grass carp in Vitro by using L-[4,5-3H] Proline,L-[2,3,4,5-3H] Tyrosine as tracers and adopting the in-vitro perfusion technique.The experimental results showed:1. Amino acid perfusion concentration had influenced remarkably absorption velocity and the absorption velocity had a positive correlativity with amino acid perfusion concentration.For C.auratus,The transmural velocities of L-Tyr were 0.387umol.g^-1(gut).min^-1,0.153 umol.g^-1(gut).min^-1 and 0.087umol.g^-1(gut).min^-1 respectively at the 10 min along with the perfusion concentration of L-Tyr changing from 2.5mmol.L^-1,1.5mmol.L^-1 to 0.5mmol.L^-1; For Grass carp,they were 0.047umol.g^-1(gut).min^-1,0.025umol.g^-1(gut).min^-1and 0.016umol.g^-1(gut).min^-1 respectively. The transmural velocities of L-Pro for C.auratus were 0.876umol.g^-1(gut).min^-1,0.426 umol.g^-1(gut).min^-1 and 0.078umol.g^-1(gut).min^-1 respectively at the 10 min as the perfusion concentration of L-Pro varied from 10.0mmol.L^-1,5.0mmol.L^-1to 1.0mmol.L^-1;For Grass carp,they were 0.302umol.g^-1.min^-1,0.255umol.g^-1.min^-1and 0.019umol.g^-1.min^-1 respectively. C.auratus was remarkably higher than Grass carp in the transmural velocity of the two amino acids(â–³S>LSD0.05),expecially...