The Preparation, Analysis And Induced Resistance To Grapes Of Oligosaccharides

Oligosaccharides derived from epiphyte play an important part in regulating plants'growth, development and induced resistance. In the research, oligosaccharides are derived from cell wall of Alternaria alternate and Botrytis Cinerea which are the main pathogen found on the grapes and the monosaccharides in the oligosaccharides has been analyzed. Aso I have researched the resistance to red earth grapes using the oligosaccharides. The research has provided the way to analyze the monosaccharides in the oligosaccharides and enriched the base theory.The fermentation conditions and medium optimization are taken on in the experiment. The best composition of the fermentation medium is as follows: glucose 3%, potato juice 4%, KNO₃ 0.1%, KH₂PO₄ 0.1%, MgSO₄·7H₂O. The improving formation conditions contain: Temperature 26℃, liquid medium quantity 100mL, pH 5.5, waving bed's rotate speed 120 r/min.The biological activity of the oligosaccharides is measured by using soy cotyledon. The result shows that both of the two kinds of oligosaccharides have the peak value of biological activity and the peak value is 1000μg/mL.The result of extracting elicitor from cell wall of Botrytis cinerea and Alternaria alternate shows that the essential components are saccharides and protein. There is 24.92% neutral saccharide, 0.21% reductive saccharide and 20.01% protein in the cell weight of B.c oligosaccharides. There is 21.68% neutral saccharide, 0.13% reductive saccharide and 18.91% protein in the cell weight of Alternaria alternate.The developed method has been successfully applied for the analysis of mono- saccharides in the two kinds of oligosaccharides. high performance liquid chromatography (HPLC) analysis showed that the oligosaccharides consisted of fructose, D– mannose, glucose, D–galactose and D– arabinose. The proportion of the five monosaccharides in Alternaria alternate oligosaccharides is 3:5:10:4:1 and the proportion of the five monosaccharides in Botrytis cinerea oligosaccharides is 1:5:3:2:2.The grapes were treated by Alternaria alternate and Botrytis cinerea oligosaccharides. The result of inoculation experiment showed that the diameter of the disease speckle and the incidence of a disease is low than the contrast. The effect of Alternaria alternate oligosaccharides is better than Botrytis cinerea oligosaccharides. But the there is no...