A Study On The Depilation Technique And The Physiology Changes Of Flower Seeds With Villus

Aimed to master the depilation technique and provide the theoretical and practical basis for improving the seed germinating rate and seedling quality, with the material of four kinds of flower seeds with villus(Gomphrena globosa var Qianrihong, Gomphrena globosa var Qianribai,'Gozania splendens var. Xunzhangju and Gaillardia pulchella var. Tianrenju), the depilation technique was studied and 3 depilation chemicals were screened. The reparatory functions and the physiological effects of PEG-6000 treatment on the Germination of villus flower seeds were also discussed in this paper, the main result are as follows:1. The treatment of HCl and NaOH concentration less than 36% were inoperative on depilation of Qianrihong, Qianribai, Xunzhangju and Tianrenju. To some extent, a given concentration of H2SO4 were active to shed seed villus.2. The optimal H2SO4 concentration for depilation is 60%, high concentration or longer treatment duration will injurey flower seeds. 1.0 gram of four kinds flower seeds treated by 20ml 60% H2SO4 reached grade A with highest germinating rate and germinating potential our experiment.3. Four kinds of shed flower seeds cultivated in sandy, vermiculite and no soil medium, germinating rate and germinating potential are as follows: soilless>vermiculite>sandy medium. The light and loose medium is good at improving the germination rate of flower seeds.4. 3 concentration of PEG-6000 were use to treat depilated flower seeds, the result showed that PEG-6000 decreased the damage of depilation chemicals to the flower seed and 20% PEG-6000 can improve the germinating rate and germinating potential of shed flower seeds. Compared with CK, the germination rate and germination potential increased obviousely: Qianribai 9.3%,14.7%,Qianrihong 13.6%,18.1%,'Xunzhangju'44.8%,22.2%,'Tianrenju'32.7%,13.7%.5. The physiological changes of 20% PEG-6000 treated depilated flower seeds was measured, the results showed that the treatment increased activity of superoxide dismutase (SOD) and catalase (CAT), decreased MDA content and electrical conductivity, enhanced anti-oxidation and seed germinating rate.