Proteome Analysis Of Leaves Of Slow-Rusting Variety, "Chuanmai 107", Inoculated By Wheat Stripe Rust (Puccinia Striformis)

Wheat stripe rust is caused by the basidiomycetous fungus Puccinia striiformis f.sp. tritici and is the most destructive disease of wheat. Use of resistant cultivars has become the major means of controlling stripe rust that is most effective, economical and safe. Because of development of the new physiologic races and simplification of cultivars, most vertical resistant cultivars have become susceptible within only a few years. So the researchers attach importance to utilize horizontal resistant cultivars increasingly. Slow-rusting variety, Chuanmai 107 and so on, as a horizontal resistant cultivar, displays very good effect on controlling wheat rust and holding the yield. We choose Chuangmai 107 as the experimental material. Through proteomic analysis of the changes of proteins in slow-rusting variety,"Chuanmai 107", we want to find some specific resistance-related proteins.The changes of proteins in two wheat varieties,"Chuanmai107"and"80 - 8", caused by the inoculation of Puccinia striiformis f.sp. tritici were analyzed using 2-dimensional electrophoresitic technology. 20 protein spots resolved on 2-D gels were found to be differentially expressed after analyzing Coomassie -blue stained gels and silver stained gels of the soluble protein samples from the control and disease leaves 7 days post inoculation. These spots were then excised from gels stained with Coomas-blue and analysed by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Peptide mass fingerprinting (PMF) of 20 proteins were submitted to NCBI and Swiss-prot databases for protein identification and homology analysis. We identified 9 proteins. Two were ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (Rubisco LSU) , and one was Ribulose bisphosphate carboxylase small chain clone 512 (RBS3_WHEAT). The remaining six were identified as cold-responsive LEA/RAB-related COR protein, manganese superoxide dismutase (MnSOD), glutathione transferase, caffeic acid O-methyltransferase (COMT), isocitrate dehydrogenase (IDH) and RecA bacterial DNA recombination protein; Rad51, N-terminal respectively. Among above, COMT was a pathogenesis-related protein.Proteome analysis of slow-rusting variety,"Chuanmai 107", may provide theoretical principle for elucidating the resistant mechanisms of slow-rusting variety. Proteome will...