Preliminary Studies On Differences Of Related Characteristics Between Perfact Flower And Impefect Flower And Protemics In Japanese Apricot

Japanese apricot(Prunus mume Sieb. et Zucc.)originated in China and is classified into Rosaceae Prunus. It was speciality tree of semi-tropical China. It can be divided into fruiting mei and flowering mei. The history of fruiting mei lased three thousand years. In this paper, related characters of several cultivars in perfect flower and imperfect flower were surveyed, the main nutrients were identified, different periods and different organs of proteins between perfect flower and imperfect flower were studied by 2-DE. The results are as follows:1. There was significantly difference between pollen quantity in different cultivars. The pollen quantity of perfect flower and imperfect flower in the same cultivars was significantly difference too.But the pollen viability of perfect flower and imperfect flower had less difference The fruit setting rate decreased with the increase proportion of imperfect flower, and the relativity between perfect flower and pollen viability was not relative.2. The content of nitrogen in perfect flower was greater than imperfect flower, but content of phosphorus was opposite. The content of potassium in perfect flower was greater than imperfect flower in the stage of young bud, and it was opposite in the stage of blossom.3. We use two-dimensional electrophoresis of proteomics to analyse the different proteins in perfect flower and imperfect flowerthe in the different stage of young bud, mature bud and the flower of blossom. The result showed that there were five special proteins and some different proteins. There were several different expression proteins in sepals, petals and stamen of two kinds of flowers.4. The special protein and different proteins in three different period of perfect flower and imperfect flower were analysed using MALDI-TOF-MS. There were three different proteins in the stage of young bud and five different proteins in the stage of young bud and four different expression proteins in the stage of flowers in blossom. The results showed that these proteins were related to Glucose metabolism, Starch metabolism and photosynthesis.