The Cell Culture Of Mythimna Separate And Cytoxicity Assay Of Six Natural Products Against The Cells

1. The cell cultureThis paper researched on the primary culture of embryo cell, blood lymphocyte, fat body cell and the midgut epithelium cell of Oriental Armyworm. The results were as follow: Embryo cell: Two different types of cells were developed from eggs of the armyworm, oval and shuttle shape cells. To the oval cells, after being culture for ten months, the cell began to passage, which appears in oval shape and attached. At present they had passaged to the third generation. To the other ones, being cultured for four months, which appeared in shuttle shape, the length of the single embryo began to prolong and divided into fan-shaped and diffused around after five months. Seven months later, the small and bright single cells can be found around the cellular bundles.Muscle cell: The muscular fibers are looser, and there were many muscle satellite cells separated from the fibers. The transverse striation over the Myoblast is arranged alternatively and the plasma membranes were integrated. After attached, a lot of muscle satellite cells turned round shape into shuttle, and their somas were plump. Those cells were acicular and thin at the two terminals and in rod shape and the muscle satellite cell division can be observed.Fat body cell:, The fat body cell appeared alveolar and diffused around. after five days. One month later, some small ovoid cells appeared which arranged closely. The small cells diffused around fifty days later,. The cells were spread at the bottle bottom and began to passage. three months later. After that, they grew slowly and assembled in groups.The midgut epithelium cell: Two different type cells were found. columnar and shuttle shape cella. To the columnar cells, become thick after three months and in columnar shape. they developed into fan-shaped and dissociate around four months later. With their concentration increasing, they turned into fanlike. To the shuttle cell, the single cell became clarity gradually into shuttle crystal after two months. Then the cell divided into clustered and the single cells turned into tufted gradually after three months. Five months later, some single cell separated from the large cluster and attached.Hemocytes cell: The dense of the cell is decline, and a few cell became bright after two...