Study On The Podophyllotoxin Analogues Content Of Sabina Vulgaris Ant.in Different Ecological Environment Condition

The evergreen prostrate shrub Sabina vulgaris Ant. (syn.: Juniperus sabina Linnaeus) belonging to the genus Sabina ( family Cupressaceae) is widely distributed in the northwest of China. Much attention has been paid to chemical constituents, bioactives, biotic, ecological and physiological characteristics of this plant. One type of mainly secondary metabolites of the plant, podophyllotoxin and its congeners exhibit pronounced biological activity mainly as strong antiviral agents, antineoplastic drugs, and as insecticides. However, the influences of environment on the content of podophyllotoxin in S. vulgaris still remain unclear. In order to provid scientific basis for the further rational utilization of the S. vulgar and podophyllotoxin., therefore, in this paper compared the contents of podophyllotoxin and deoxypodophyllotoxin of Sabina vulgaris of different habitats, varieties and plant parts. And the relationship between the ecological factors and the podophyllotoxin and deoxypodophyllotoxin content in the plant were studied, using supercritical fluid extraction (SFE) and high performance chromatography (HPLC), and the results were described as following:1. The conditions for extraction of podophyllotoxin and deoxypodophyllotoxin from S. vulgaris with SFE were studied. The podophyllotoxin and deoxypodophyllotoxin content were analyzed by HPLC with PAD detector. The extracting efficiency was evaluated by 3 indexes, i.e., total compound extraction ratio, podophyllotoxin extraction ratio and deoxypodophyllotoxin extraction ratio. Using static extraction followed by dynamic extraction methods, the effects of different parameters, such as pressure, temperature, modifier volume, sample size and extraction time, on the supercritical fluid extraction (SFE) of podophyllotoxin and deoxypodophyllotoxin were investigated. The results showed that, under a pressure of 6000psi, temperature 60℃, flow-rate of CO2 30mL/g, sample size of 0.28mm size and static extraction time of 15 min, use the chloroform as modifier, the quantity is 3.0mL/g, extraction was more selective for the podophyllotoxin and deoxypodo- phyllotoxin .2. The contents of podophyllotoxin and deoxypodophyllotoxin of S. vulgaris leaves, stem and berries were examined. The results showed that the contents of podophyllotoxin and deoxypodophyllotoxin were decreased in the following order: leaves >berries>stems. It...