The Primary Studies On The Insecticidal Ingredients From The Branches And Leaves Of Sabina Vulgris Ant.

The insecticidal activity of Sabina vulgaris Ant. was fovmd and reported by the Research and Service Center of Biorational Pesticides, NWSUAF, subsequently, three insecticidal ingredients-tepineol-4-ol which is isolated from the essential oil, deoxypodophyllotoxin from the fruit and podophyllotoxin from the high polar extract of leaves, were isolated.The work of this treatise basing on the low polar extract of leaves, which is not studied before,looks for other insecticidal ingredients from Sabina vulgris Ant..The main results are as follows:The extraction, isolation and identification of the insecticidal constitution were studied. The powdered leaves of Sabina vulgaris Ant. was extracted with EtOH(95%), and the solvent was removed. The EtOH extract suspended by distilled water was allocated to four different polar solvent-Petroleum ether, Chloroform, Ethyl acetate and Butanol by Counter Current Distribution, the four extracts were concentrated by removing the solvent. The activity was tested by the larva of Mythimna separatea W.. The results idicated: all extracts ,except butanol extract, had better repellency active; in addition, chloroform and ethyl acetate abstracts had better toxicity. Mythimna separatea W. was used as test insect, and active guiding method was applied to test the activity of Petroleum ether abstracts. After four normal pressure pillar chromatographys, three active materials were gained, which were Y2, Y7, Y8. The melting point test and TLC test make clear that the active materials were pure counpond. Comparing to the standard chromatography chart, UV, MS, 1HNMR data make clear that the three materials were p-steriol (Y2), docosanoic acid(Y7), dioctyl acid(Y8).The insecticidal activity of p-steriol to several insects was investigated. The result showes it has higher antifeeding activity to Pieris rapae L.and the activity of 48h is 61.57%,with the dosage of Img/mL. It also has antifeeding activity to Mythimna separatea W. But it showes no any activity to Aedes albopictus S. and Plutella xylostella L.under tested dosage. Comparing to ethanol abstracts, pertroleum ether abstracts and various distillations, the p-steriol had different result. So some other ingredients may exist in Sabina vulgris Ant. or have interplay action with other materials.The paper told that the P-steriol was one of the active materials of Sabina vulgris Ant. and the ingredients of insecticide materials in Sabina vulgris Ant. were complex. So the further research on active materials and the interplay of which was very necessary.