Study On Bdellovibrios Infacting Fish-pathogen Bacteria

Bdellovibrio are predators of other bacteria,especially gram-negative bacteria. The prey range of Bdellovibrio is very wide .It no only can lyse some common bacteria,for example E.coli salmonella,but also the fish pathogenic strains Aeromonas Proleus and Pseudomonas. They lyse the pathogenic strains stronger than others.The study of Bdellovibrios is very slow. The early study of Bdellovibrios focused on their biological characteristics and physiology characteristics. When the Genomic Perspective of Bdellovibrio Bacteriovorus HD100 was reported, the study of Bdellovibrios happened to develop advanced rapidly, and also the Micro Ecology research . Researchers in China mostly make the Bdellovibrio applied in aquatic products.Four pathogenic bacteria named N1,N2,N3 and N4 were originally isolated from moribund Silurus meridionalis in an outbreak of hemorrgagic disease at a fish farm in Meishan. Healthy Silurus meridionalis were infected by injection and immersion with four strains of the isolated bacteria and the 4 strains induced 100% mortality by the Koch's postulate. The strain N3 is identified as Proleus strain. Eleven strains of Bdellovibrios are separated from the pound sullage and soil which is from Chengdu TongWei Aquatic Science & Technology Co. Ltd., the sewage which is from Chengdu sewage farm, and the soil which is from Ya'an. Meanwhile the separated strain(Proleus strain) and Aeromonas hydrophila were selected as host. Then there are a series of study on the biological characteristics of Bd-tw1 Bd-tw3 and Bd-tw7. The prey range of Bdellovibrios were very wide . It can lyse the gram-negative strains Aeromonas hydrophila , N1, N2, N3, N4, Proleus strain and...