The Construction Of ELISA Method To Test Plasmodiophora Brassicae

Plasmodiophora brassicae is a kind of world-wide spread botanical disease. Most of the hosts are general vegetables of our daily life that we should depend on. Some of them can produce oil we eat(rape) and others are vegetables on our tables (cauliflower,cabbage,Chinese cabbage), what's more, it can infect some kinds of Cruciferous weeds. Every year the disease may cause great lose of some vegetables and endanger the safety of our society.Plasmodiophora brassicae is an obligatory parasitic fungus. It can not be cultured outside certain plant organism, which make it is very difficult to do research on them. Now there is no effective method can controll the happen of the disease.Accout to the upper reason, we expect to get a method which do help to chech infected soil quickly,cheap and simply.The result got are listed as follow:1,Optimized the purification method of Plasmodiophora brassicae spores by concentration rotation. The raw suspend of spores were harvested and the best match of sugar concentration is mixtured by the same amount of 30, 40, 50 and 60% sugar solutions.2, It is the first time that ultrasonic method was applied to purify spores. Under the check of microscope, the surface of zoospores became very clean and the conj action on the surface was departed.In the research, the purification method was optimized, and under the best condition, the power of ultrasonic is 345W, treated time is 10 min, pH 5.5, the concentration of spores is about 7×10~8 and 0.5% DMSO is added. Under the condition, when the number of spores rises to the highest point, the...