Study Of Phylogenetic Relationship Of Cerambycidae, Gene Code Building And Numts

Some fragment of mtDNA of samples were respectively sequenced,and these samples including individuals from different subfamilies of Cerambycidae.The relation ships of these samples from different subfamilies and the reasons brought numts were studied. The results were an follows:We sequenced Cytb DNA and 16S rRNA of the samples from different subfamilies and builded phylogenetic tree.The tree was builded according to the differences between the sequences of the samples.We studied the classification of these samples.The result of the analysis of Cyt b DNA sequence indicated that the Tetropium castaneum and Pachyta bicuneata were grouped in a same clade of the phylogenetic tree and subsequently integrated the clade of the Anoplophora glabripennis.After they integrated at same joint,the three subfamilies finally meet Trichofreus compestris and Megopis sinica(White) at the other joint.The distance from joint of Megopis sinica(White) was farer than Trichofreus compestris. According to the result we can included that the relationship of the five subfamilie as follows: Prioninae→Cerambycinae→Lamiinae→Aseminae→Lepturinae. The tree which builded according to the sequence of 16S rRNA indicated that the Tetropium castaneum and Pachyta bicuneata were grouped in a same clade of the phylogenetic tree and subsequently integrated the clade of the Apriona germari Hope . After they integrated at same joint,the three subfamilies finally meet Trichofreus compestris and Megopis sinica(White) at the other joint.Also,the five subfamilies relationship as follow: Prioninae→Cerambycinae→Lamiinae→Aseminae→Lepturinae.Result of the two studies jointly prove the same relationship of the five subfamilies.We sequenced 30 samples of Cerambycidae,1 samples of Buprestidae and 1 samples of Chrysomelidae. We studied the feasibility of the building of the gene code .The result indicated that the difference of the sequence was more larger within than between species. So we believed that this fragment of mtDNA COⅠcan be used as a marker to build the gene code of all individual of Cerambycidae. We inspect the delicacy of this PCR system,and the result proved that the system had a high veracity and stability. The number of species in this study was inadequate and important species and quarantine species were should be sequenced in next study.This study will provide scientific base of the inspection, genetics and phylogenesis.We found that the sequences of COⅠ-2 of Anoplophora glabripennis form different area were divided into two group on base of the difference of sequence.After sequenced the Anoplophora glabripennis form Hebei province with the long PCR,we believe that the numts was existed in one group.The reason of appearance of the numts were analyzed then we found the possible reason.We believe that the conserved primers and the low content of mtDNA were the most possible reasons.