Slow Rate Cryopreservation And Vitrificated Cryopreservation Of Chicken Embryonic Primordial Germ Cells

This paper focused on the primordial germ cells (PGCs) of chicken from gonads at stage 19 and stage 28. The PGCs were isolated by Ficoll density-gradient centrifugation. Then, the PGCs were froze respectively in six kinds of freezing medias under the slow rate cryopreservation process and another six kinds of freezing medias under vitrification process. The slow rate cryopreservation process was that, equilibrating the PGCs suspension in the freezing vials at 40C for 45~60 min, enwrapping the vials with cotton, holding the enwrapped vials on the surface of the liquid nitrogen overnight and then plunging directly into liquid nitrogen. The vitrification process was that, equilibrating the PGCs suspension in the freezing vials at 40C for 5 min, holding the vials on the surface of the liquid nitrogen for 1 min and then plunging directly into liquid nitrogen.The six kinds of slow rate cryopreservation freezing medias were following: I : 10 % DMSO+10%FBS+DMEM ; II : 10 %EG+10%FBS+DMEM;III:5%DMSO+5%EG+10%FBS +DMEM;IV:10%DMSO +0.1mol/LSurcose+10%FBS +DMEM ; V : 10%EG +0.1mol/LSurcose+10%FBS +DMEM;VI:5%DMSO+5%EG +0.1mol/LSurcose+10%FBS +DMEM. The six kinds of vitrification freezing medias were following: I:10%DMSO+10%EG+10%PVP,II:20%EG+10%PVP,â…¢:20%DMSO+10%PVP,â…£:10%DMSO+10%EG+0.5mol/LSurcose,...