Preparation Of The Polyclonal Antibody Against Reticuloendotheliosis Virus-gp90 And Expression Of The Gp90 In Insect Cells

The reticuloendotheliosis viruses (REVs) are a group of oncogenic and immunodepressive type C avian retroviruses which cause reticuloendotheliosis. REVs are known to cause economically important immunodepression in infected chickens, and sometimes, found as contaminants of Marek's disease and fowlpox vaccines. REV is associated with sporadic outbreaks of chronic neoplastic disease in turkeys and can cause significant losses in commercial turkey flocks. Although accurate data on the economic significance of REV-associated diseases is not available, the oncogenic potential of these viruses, their ability to cause immunodepression, and their presence as contaminants in poultry bioproducts is clear.The envelope glycoprotein of reticuloendotheliosis viruses (encoded by the env genes) gp90 is the major immunogenic protein, known to be associated with virus neutralization, has continuous and discontinuous isotopes. There were dates showed that the sequence analysis of the env genes of the various strains of REV shared 92% identical to each other at the gene level. gp90 may be responsible for the variation, virulence, and pathogenicity of the reticuloendotheliosis viruses.In this thesis, Envelope glycoprotein gp90 gene of reticuloendotheliosis virus (REV) was digested by restriction enzymes from expressing vector pGEX-6p-1-gp90, and sequenced. The expressed fusion protein was identified by SDS-PAGE, and found to be 64 kd band in size. The GST-gp90 fusion protein was existed in inclusion. The serum from the mice immunized with specific fusion protein excised from gel showed positive to CEF infected with REV in indirect immunofluorescence assay (IFA). The...