Study On Humoral Immunity And Cellular Immunity In Piglets With Cysticercosis Cellulosae By Artificial Infection

Cysticercosis cellulosae is a parasitic zoonosis, which endangered severely the poricine production and the health of human being. It's pathogeny is a kind of complicated eukaryotic organism and consists of the four developments of Teania solium, Oncosphere and Cysticercus cellulosae, which infected and parasitized repeatedly between pig and human. At present, studies on many aspects, such as the infection and immunization, immunization and immune escaping, as well as immune prevention, et al.have made agreat progress on the individual, cell and molecular levels of parasite and host in the world.The relationship among the component, structure and development form of parasite and infection and immunity have been clarified. But this is only the first step on preventing and controlling the disease, and still has a long way to go.SephadexG-200 chromatographic technology was performed for purifying cysticercosis cyst fluid rude antigen (CF), obtained 2 chromatographic antigens (CF1.CF2). Detected by ELISA test respectively,the results showed that CF₁ antigens had high specificity, good sensitivity, high stability and repeatability can be immune diagnostic antigens of cysteicercus cellulosae diseased swine IgG again. Improved the original method in laboratory determined, defined the best condition for every stage, furthermore it offered Mirror and reference to epidemiologic survey of Cysticercosis cellulosae.Objective: Objective: to explore the dynamic changes of level of antibody serum in peripheral blood of piglets with Cysticercosis cellulosae by artificial infection. Methods: All piglets were grouped 2 at random, infected group and contract group, the antibody in peripheral blood of piglets was detected by ELISA, optimized the original method in laboratory determined at experiment I, after 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80 days infection. Results: The dynamic changes of level of antibody serum were fluctuated with augment of days injection. And the earliest doubtful masculine antibody was shown up after 10 days infection(only one piglet and OD value was lower than critical value); two piglets were masculine after 15 days infection, all piglets were shown up positive by ELISA after 20 days infection, the peak time is at 40 days post infection. Then valence of antibody is lasting to step down with augment of days post infection, but it still retains high level.