Study On Immunoenhancement Mechanism And Screening On Chinese Medicine Prescriptions Of Enhancing The Chicken Immunity

In this study, on the basis of invigorating vital energy and enriching the blood, the three different Chinese herbal medicine prescriptions were organized with Astragalus, Epimedium, atrctylodis macrocephalae, Raidix Ophiopogonis and so on. These three prescriptions all had the effect of immune enhancement. In order to determine the effect of the prescriptions on the immune enhancement of the chickens at high, middle, and low doses, 500 seven-day-old chickens were randomly divided into ten groups, 50 in each group. The chickens in groups 1 to 9 appended 0.5%, 1.0% and 1.5% of each prescription into the stoyer. The chickens in group 10 fed stoyer only. The chickens in groups 1 to 9 were treated with successive medication for 15 days. On the 7th day and the 21st day the chickens were vaccinated. Ten chickens were selected randomly from each group. At the 14th, 21st, 28th, 35th, 42nd, 49th, 56th and 63rd days the contents of HI antibody to ND were determined. Bursa of fabricius and spleen were collected on 21st, 28th, 35th and 63rd, and the bursa of fabricius and spleen indices were determined.The results indicated that prescription I at 1.0% dosage and prescription II at 1.0% dosage could increase the level of HI antibody, and from 35th day to 63rd days it kept the level of HI antibody at 8 or 9 log2s. Compared with control group, prescription III at 1.5% dosage group increased the level of antibody significantly. In 21 and 35 days old each index had significant difference while there was no difference in other ages compared with the control. The three different prescriptions could increase the level of antibody and growth index of immune organs. The effect of herbal prescription I at 1.0% dosage was the best.In order to study the immunological enhancement mechanism of the herbal prescription I, 80 ICR mice were used. The animals were randomly divided into 8 groups, i.e.: (l) three (heavy, middle and slight) dose groups of prescription I in normal mice; (2) three (heavy, middle and slight) dose groups of prescription I in immunosuppressive mice; (3) NS control groups in normal and immunosuppressive mice. The immunosuppressive mice models were established by intraperitoneal injection of hydrocortisone (Hy). All groups of mice were administered with the herbal pulvis by gavage for six days consecutively. The thymus and spleen indices, the clearance rate of charcoal particles, the function of AFC, the level of serum hemolysin, and CD4+/CD8+T cell ratio in serum of each group were observed. The results showed: Certain dosage of herbal prescription I significantly improved the thymus and spleen indices (p<0.05), and significantly improved the clearance rate of charcoal particles in immunosuppressive mice compared with that in NS control group (p<0.01). There were extremely significant effects on the function of AFC and the increase of serum hemolysin level in immunosuppressive mice (p<0.01). Herbal prescription I had certain promotion and enhancement to the CD4+/CD8+ T cell ratio in peripheral blood of normal and immunosuppressive mice (p<0.01). All results above indicated that herbal prescription I had certain enhancement to the nonspecific immunity, humoral immunity and cellular immunity function of mice.