Studies On Isolation And Pathogenicity Of Fusarium From Highland Barley Kernels In Tibet

The diseases of highland barley caused by fungal especial, Fusarium spp. have been a serious problem in highland barley-producing areas in Tibet. The disease was caused by Fusarium spp accounts for one third of all fungi diseases in highland barley. This disease not only cause highland barley yield decrease and quality reduction, but also arose human diseases by the toxic it secreted, such as cancer, Kaschin-Beck disease, Keshan disease. For offering theoretic bases to prevent and control these highland barley diseases and improving its quality and quantity, eliminating the human diseases which was caused by the toxic secreted by the fungi, highland barley samples were collected from five Tibet areas in 2004-2005. Fusarium species fungi were isolated from these samples and identified through morphological and molecular biological characteristics. The pathogenicity of all isolates and the biological characteristics of isolates Hb39 were determined in vitro and in vivo. The main results are listed as follows:A total of 61 isolates of Fusarium were isolated and identified from 5,000 highland barley kernels of Lhasa in Tibet. The isolates of Fusarium in kernels were up to 1.22%, the most was 1.70% of Linzhi, and Rikaze was the least.The 61 isolates of Fusarium were identified to be nine Fusarium species according to classification standards of literature and molecule biology method. There were Fusarium avenaceum(60.66% isolates), Fusarium tricinctum(22.95% isolates), Fusarium moniliforme var. subglutinans(6.56% isolates), Fusarium nivale(1.64% isolates),Fusarium flocciferum(1.64% isolates) , Fusarium solani(1.64% isolates) , Fusarium stilboides (1.64% isolates) , Fusarium arthrosporioides(1.64% isolates) , Fusarium spp.(1.64% isolates). And F. avenaceum was the predominant species of Fusarium in highland barley in Tibet.The germination of highland barley seeds by isolated was tested, the result showed that the isolate Hb16 could reduce significantly the germination of seeds, the rate of germination was only 29.14%. Also, the isolates could influence the growth of highland...