The Effects Of Goat Fetal Tissue Extract On Proliferation And IL-4 Secreting Of PBMC Of Pig

Through Detecting the effect of FTE on Proliferation and IL-4 secreting of PBMC of pig by MTT essay and ELISA essay respectively to illuminate the effect of fetus on regulation of immunologic balance in local uterus. Results obtained as follow:(1) FTE can markedly stimulate the proliferation of PBMC, and the stimulating effect of FTE on PBMC proliferation is related with fetal ages and FTE protein concentrations. The proliferation effect of FTE becomes weaken with the increasing of fetal ages; The optimal effect of FTE on proliferation of PBMC is when FTE protein concentration is 1×10^-4 mg/mL～1×10^-5 mg/mL.The stimulation effect will decrease when concentration becomes higher or lower. FTE can inhibit the effect of PHA-P on proliferation of PBMC, the inhibiting effect of FTE is related with it's protein concentration, and it becomes stronger with the increasing of fetal ages.Maternal uterine vein serum(MUVS) can markedly stimulate the proliferation of PBMC too.The stimulating effect of it is accorded with FTE. MUVS can also inhibit the effect of PHA-P on proliferation of PBMC.(2) FTE can stimulate PBMC to secrete IL-4. The IL-4 level stimulated by FTE becomes higher with the increasing of fetal ages.MUVS can stimulate PBMC to secrete IL-4 too, and the stimulating effect of it is accorded with FTE at the same pregnant period(P>0.05).These results indicated that through affecting the activity of lymphocyte of local uterus, FTE can make the secreting effect of Th2 cytokine become stronger, and that makes the immunologic balance of Th1/Th2 in local uterus deviate to Th2 effect, this change is profit to pregnancy, and make the fetus avoid from maternal rejection.