The Effect On Behavior And Synthesize Of Vitellin Of Diamondback Moth, Plutella Xylostella L. Of Polyamines

Polyamines, such as putrescine, spermidine and spermine, are ubiquitous physiological cations. They occur in almost all living species, both prokaryotes and eukaryotes. Polyamines are essential for the synthesization of large biologic molecule, cell growth and differentiation. They can also regulate the physiological process of insect such as growth and development et al.. Whereas the regulation mechanism is unclear because of the complexity of the physiological process of insect and the metabolism of polyamines. So, studying the effect on behavior and synthesization of vitellin of diamondback moth are essential for understanding the regulation function of polyamines on the physiological process of insect. In this study, we studied the function of polyamines in the behavior of diamondback moth by the behavior responses, electroantennogram responses and the changes of antennal proteins; studied the effect on the metabolism of polyamines and hormone in the base of determination of polyamines by HPLC; and studied the effect on the synthesize of vitellin diamondback moth of polyamines and hormone by electrophoresis and immune technology. The main results as follows:1 The effect on the behavior of diamondback moth of polyaminesBoth unmated and mated, the diamondback moth adults had an obvious orientation response to those three polyamines (spermine, spermidine, putrecine) compared with distilled water; but no difference among the three polyamines. Except that unmated-males had an obvious orientation response to putrecine compared with cabbage juice and mated-male had an obvious orientation response to cabbage juice compared with spermine, the moth had the same orientation response to the three polyamines and cabbage juice.Both unmated and mated, the diamondback moth adults had an obvious Electroantennogram response to those three polyamines (spermine, spermidine, putrecine) compared with distilled water; but compared with Chinese cabbage juice some had obviously lower Electroantennogram response and some had obviously higher one. The moth had obvious Electroantennogram response to lower thickness (0.1%) polyamines compared with higher thickness (1%).The Electroantennogram response to Chinese cabbage juice (and different thickness polyamines) of diamondback moth have obviously changed after treated with 3 polyamines(spermine, spermidine, putrescine) and DFMO. And the Electroantennogram...