The Screen Of The Functional Microbial Strains And The Study On Activities Of Microbial Combination Communities

With the development of microbial technology, compound microorganism fertilizer has become one of the most popular problems now. The microbial functional community as the most important parts of compound microorganism fertilizer was the key technology in fertilizer production. In this paper, the phosphate solubilizing microorganism, nitrogen fixation microorganism and potassium dissolving microorganism have been screened, and their activities have been studied. The primary mixed fermentation experiments have been carried out to look for a combination of Microbial Community with the highest activities.11 strains of phosphate microorganism were isolated in advantage of Pikovskava phosphate dissolving medium. Among them, the Bmp5, Bmp6, Bmp7 and Fmp9 had the higher phosphate dissolving activities.In hydroponics'condition, the phosphate solubilizing abilities of the four tested strains (Bmp5, Bmp6, Bmp7 and Fmp9) in dissolving five different poorly soluble phosphates were studied with two strains (Pesudomonas fluorescens As1.867 and Bacillus megatherium As1.223) as comparison. The obtained results indicated that these single strains exhibited higher solubilizing abilities in Ca₃(PO₄)₂, AlPO4·2H2O and CaHPO4·2H2O than in FePO4·2H2O and lecithin. As an example, the amount of the phosphate solubilizing capacity of Fmp9 was 92% and 48%, respectively, higher than those of the references As1.867 and As1.223 in solubilizing Ca₃(PO₄)₂. When using CaHPO4·2H2O as the sole phosphate source, The phosphate solubilizing capacity of Bmp5 was 2.17 times higher than that of As1.223, while the phosphate solubilization capacity of Bmp6 could be as high as 785.51mg/L, 35% higher than that of As1.867, 3.43 times than that of As1.223. As1.223 showed the maximum solubilization in AlPO4·2H2O.The study of the liquid co-culture of the four strains showed that the phosphate solubilizing activities of the combinations of Bmp5+Bmp7 and Bmp6+Bmp7 decreased greatly, while Bmp5+Fmp9 and Bmp6+Fmp9 were the rather preferable combinations.The optimized culture conditions of the highest phosphate solubilizing acitivity of Bmp5 were pH in the range of 5.5⁸.0; using ammonium oxalate, glycerol as carbon source; ammonium oxalate as nitrogen source and temperature at 35℃. The...