Development Of Multiplex PCR For Diagnosing The Disease Caused By Streptococcus Suis And Cloning And Expression The Fibrinogen-binding Protein Of Streptococcus Suis Type 2

Streptococcus suis are worldwide zoonotic phathogens of many different diseases such as meningitis, septicemia, arthritis, endocarditis and may cause sudden death in pig or human, which made substantial economic losses for pig industry and became a substantial threat to human health especially those involved in the pig industry .So far, thrity-five different serotypes(type 1/2 and 1 through 34) of S.suis have been described . Of the 35 serotypes ,type 2 is the most frequently isolated serotypes from pigs with disease , but strains belonging to other serotypes such as types 1/2,1,7,9,and 14 can also caused disease . However, not all strains of S. suis type 2 are virulent, and there is variation in the virulence of those strains that are virulent. A number of potential virulence factors have been identified and partially characterized. They include extracellular factor(EF) and murimidase-released protein(MRP); suilysin(sly); a 44kDa cell wall protein; an immunoglobulin-binding protein, however, so far, the detection of S.suis has been limited to serotype 2 strains in China .To more convenient detection of specific serotypes and virulence-associated phenotypes of S.suis, PCR procedures had been developed in the assay. The PCR primers in this assay are based on the sequence of mrp, epf(epf*), and sly gene and the capsular polysaccharide biosynthesis loci(cps) of S.suis serotypes 1,2,1/2,7,9,14.Recently, a new virulence factor was identified by using a complementation approach . Based on the facts that FBPS is immunogenic in pigs and that fbps gene is present in all prevailing S.suis serotypes except for serotypes 32 and 34, FBPS is a very attractive candidate for a cross-protective vaccine against Streptococcus suis.1 Multiplex PCR assay for simultaneous detection of six serotypes of Streptococcus suisA multiplex PCR assay for the detection and identification of various Streptococcus suis strains in tonsillar specimens from pigs was developed and evaluated. In this reaction ,five distinct DNA targets were amplified. One target, based on the gdh gene,...