| Avain coccocidiosis is a cytoplastic entozoic parasite disease that causes severe economic loss in the poultry industry . The emergence of drug-resistant parasites and the drugs residues problem restricted the development of poultry industry so that a new approach to prevent avain coccocidiosis should be found. It have proved that stimulating the cell immuno-reaction of the host is an effective approach to prevent avain coccocidiosis . A prominence advantage of DNA vaccine as a new generation of vaccine is that it can induce abroad cell immuno-reaction and form long perdured immunity memory, especially the immune regulative DNA vaccine which was constructed by special antigen together with some cytokine genes has more effect and prospect in the anti- coccocidiosis practice. In this investigation we selected the TA4, MZ5-7, SO7, pEtK2 genes of Eimeria tenella antigen to construct DNA vaccine pVAX1.0- TA4, pVAX1.0- MZ5-7, pVAX1.0-SO7, pVAX1.0- pEtK2 and combined with the chicken cytokine ChlFN-γ to construct immuno-regulative DNA vaccine pVAX1.0- TA4-IFN-γ pVAX1.0- MZ5-7-IFN-y, pVAX1.0- SO7-IFN-γ pVAX1.0- pEtK2-IFN-γ. The protective effects of these vaccines were compared in the experiment of chickens against the challenge of Eimeria tenella.For convenience to construct DNA vaccine, designed special primers were used to reconstruct Eimeria tenella antigen gene TA4, MZ5-7, SO7, pEtK2 and chicken cytokine ChlFN-γ by PCR method. And pMD18-T-TA4, pMD18-T-MZ5-7, pMD18-T-SO7, pMD18-T- pEtK2, pMD18-T-IFN-γ constructed by our laboratory were used as the PCR templates.IPTG was used to induce the expression of the E. tenella TA4, MZ5-7, SO7, pEtK2 proteins by vector pET28b-TA4, pET28b-MZ5-7, pET32a(+)-SO7, pET-28a(+)-pEtK2 constructed by our laboratory. The pET28b-MZ5-7 was not good expressed as expect. Purified TA4, SO7, pEtK2 proteins was used to immune rats for E. tenella TA4, SO7, pEtK2 anti-serums.These anti-serums were used to check the expression of TA4, SO7, pEtK2 DNA...
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