| Stephania kwangsiensis Lo. is the endemic plants of Guangxi region .It is the perennial quality of the grass leaf rattan plant of Stephania terandra, produced in the north-west to south-western Guangxi. Born in the rock Hill,the limestone areas.Mainly Baise area of Jingxi,Lingyun and Napo . Civil Guangxi Zhuang nationality and other ethnic minorities are commonly used herbs, the root tuber production of Chinese medicine containing pain will be important raw materials used in clinical analgesic, sedative, eases.In recent years, Stephania kwangsiensis Lo. is destroyed by artificial factors such as logging, reserves have been declining, the massive artificial cultivation imminent. Germplasm resources are breeding material basis, the discovery and use of genetic resources is the source. Germplasm resources genetic diversity study will assess the prospects for the development of genetic resources conservation and species cultivation and provide important information. Stephania kwangsiensis Lo. Is to be researched on the genetic diversity to be protected and expanded cultivated germplasm resources to be of great significance.This study were collected Jingxi population ,Lingyun population , Napo population in Guangxi Baise area and cultivation population which from Jingxi ,Lingyun and Napo in Guangxi Botanical Garden. 4 of the leaves in populations of a total of 92。The 11 primers employed produced a total of 61 scorable amplified fragments, of which 60(98.36%)are polymorphic. Genetic diversity analysis showed that Nei's gene diversity (He)was 0.3379 and Shannon's genetic diversity index (I ) was 0.4165.Genetic differentiation mainly occurred among populations (Gst =0.1613),variation among population is 83.87%,variation within populations is 16.13%. Introduced population and three wild populations genetic identity 0.8846.The 15 primers employed produced a total of 82 scorable amplified fragments, of which 78(95.12%)are polymorphic. Genetic diversity analysis showed that Nei's gene diversity (He) was 0.3284and Shannon's genetic diversity index (I) was 0.4912.Genetic differentiation mainly occurred among populations( Gst =0.1563),variation among population is 84.37%,variation within populations is 15.63%. Introduced population and three wild populations genetic identity 0.8297.UPGMA cluster analysis was carried out using software NTSYSpc2.11s.Two dendrograms were contructed based on the ISSR and RAPD data respectively, UPGMA analysis showed that the 92 individuals were not clustered into 3 clusters corresponding to the 3 populations with the ISSR data and RAPD data. Variation among population was the higher levels and variation within populations was not significant.ISSR and RAPD received by similar coefficient that the correlation coefficient between the higher the relevance that the application of these two technologies on the analysis of genetic diversity in Guangxi to be highly consistency.Based on the sets of similarity parameters calculated with ISSR and RAPD data respectively (r=0.8925>r=0.01),indicating that the two methods were consistant and able for detecting genetic diversity in Stephania kwangsiensis Lo.Research shows that ISSR and RAPD are two ways of populations in the effective detection of genetic structure, Stephania kwangsiensis Lo. has a higher level of genetic diversity, three populations in the wild of genetic diversity does not differ greatly from that of Stephania kwangsiensis Lo. transplant , to a large extent maintain the genetic diversity of populations. Introduced protect more successful. At the same time we should also pay attention to the protection of wildlife and Stephania kwangsiensis Lo. to be used, if possible, to be...
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