Proteomic Analysis Of Salt-Tolerant And Salt-Sensitive Tomato Under Salt Stress

The protein expression patterns of leaves from two wild tomato species Lycopersicon peruvianumLA111, Lycopersicon pennellii LA716 and one cultivar Lycopersicon esculentum Mill'XF 98-7'were compared when they grew under 150mM NaCl stress for 14days. Tomato leaves were suspended in precooled TCA- acetone solution, dissolved in lysis buffer, and rehydrated in rehydration buffer. After IEF, equilibration and SDS-PAGE electrophoresis, the two dimensional electrophoresis maps were obtained. Captured by ImageScaner 5.0 and analyzed by ImageMaster 2D Platinum 5.0, each gel map showed more than 800 protein spots reproducibly identified. 16 protein spots were up-regulated and 11 were down-regulated in Lycopersicon peruvianum LA111; 15 were up-regulated and 18 were down-regulated in Lycopersicon pennellii LA716; 28 were up-regulated and 17 were down-regulated in Lycopersicon esculentum Mill'XF 98-7'. 20 protein spots which expressed most differently were analyzed by MALDI-TOF-MS and Mascot software. In Lycopersicon peruvianum LA111, carbonic anhydrase, threonine deaminase and erredoxin-- NADP(+) reductase were identified up-regulated; chaperonin-60 beta subunit, 2-oxoglutarate-dependent dioxygenase homolog and alpha1-tubulin were down-regulated. Chitinase and phosphoglycerate kinase-like were changed in Lycopersicon pennellii LA716. RuBisCO large subunit, ATPase alpha subunit, UTP--glucose-1-phosphate uridylyltransferase and ascorbate peroxidase were up-regulated proteins in cultivar Lycopersicon esculentum Mill'XF 98-7', while...