The Characterization Of Chimeras Of Tuber Mustard (Brassica Juncea) And Red Cabbage (Brassica Oleracea)

In this study, the morphological characters of chimeras of tuber mustard and red cabbage were described, the acid phosphatase isozyme banding patterns and the molecular marker were analyzed. This research provided useful information for the further utilization of chimeras of tuber mustard and red cabbage on breeding, the studies of ontogenesis and interactions between different cells or tissues. The main results were summarized as follows:1. The chimeras were propagated by using nodal segments as the explants on 1/2MS medium supplemented with 0.2 mg/L BA, 3% sugar and 0.8% agar. Four different chimeras were obtained. Based on the morphological characters and leaf sections, the cell layers constitution (LI-LII-LIII, T=Tuber mustard, C=Red cabbage) of them were determined, which were TCC, TTC, TCC+TTC and TCC+CCC, respectively.2. The morphological characters of chimeras were studied. The results showed that the chimeras didn't simply present the characters of tuber mustard and red cabbage, respectively. Many characters of them such as flower and leaf were intermediate between two parents. The leaf sections showed that the epidermis of chimeras were consistent with tuber mustard (half on TCC+CCC), the spongy tissue of them were consistent with red cabbage. The palisade tissues of TTC was similar to tuber mustard while TCC, TCC+CCC and TCC+TTC were similar to red cabbage3. The acid phosphatase isozyme of chimeras was analyzed by polyacrylamide gel electrophoresis. Specific band of tuber mustard and red cabbage respectively were detected which all the chimeras shared. The banding patterns of chimeras showed distinct difference based on them. TTC and TTC+TCC shared the another specific band of tuber mustard, but TCC and TCC+CCC didn't showed.4. DNA analysis of chimeras was performed by PCR method by using specific primers, The tuber mustard-specific band and the red cabbage-specific band were presented and they were detected in all chimeras. Furthermore, a unique band close to red cabbage-specific band for chimeras was detected.