| 2-pyrimidinyloxy-N-arylbenzylamme derivatives is one kind of novel herbicide precursor compound, which originated by China patent. ZJ0273 is a new herbicide derived from this compound. Researching the lethal mechanism of ZJ0273 can guide us using ZJ0273 scientific, safely, and reasonablely, which may help to find out the structure-activity relationships of the same compounds and provide provide some clues for further development of such new novel herbicides as well.In this paper, we studied in the effect of leaf cells ultrastructure, antioxidation system in plants and chloroplast fluorescence characters after treated by ZJ0273. The main results as follows:1. Study of the leaf cells ultrastructure of Malachium aquaticum after treated by ZJ0273 indicated the chloroplasts was destroyed severely, the number chloroplasts decreased, chloroplasts membrane disappeared and the structure was destroyed absolutely.2. Study of the antioxidation system in different plants after treated by ZJ0273 indicated that both the antioxidation system in Brassica campestris and Malachium aquaticum was influenced. In Brassica campestris and Malachium aquaticum, the activity of POD,the producing rate of superoxide anion and MDA were rised. While, the activity of SOD in Brassica campestris had increased faster than Malachium aquaticum. The maximum of superoxide anion relatively increase rate in Malachium aquaticum is 1.8 times than which in Brassica campestris; The maximum of MDA active relatively increase rate in Malachium aquaticum is 3.28 times than which in Brassica campestris. The maximum of SOD active relatively increase rate in Malachium aquaticum is half than which in Brassica campestris. The maximum of POD active relatively increase rate in Malachium aquaticum is 19% than which in Brassica campestris.3. Study of the chloroplast fluorescence character in different plants after treated by ZJ0273 indicated that in the lower concentrations treatment, the Fo, Fv/Fm, Fm, Fm/Fo, qP, qN, Fv/Fo had little different in Brassica campestris. In the higher concentrations treatment, the Fo, qN were increasing and Fv/Fm.Fm, Fm/Fo, qP, Fv/Fo were declining. Along with time processing, the chloroplast fluorescence parameters can revert to the control level ultimately. In the lower concentrations treatment, the Fo, qN were increasing and Fv/Fm, Fm, Fm/Fo, qP, Fv/Fo were declining in Malachium aquaticum. The chloroplast fluorescence parameters can not revert to the control level ultimately. In the higher concentrations treatment, it induced the death of Malachium aquaticum. |