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Nematicidal Activities Of Several Plant Extracts Against Ditylenchus Destructor And Separation Of Active Ingredients

Posted on:2008-04-05Degree:MasterType:Thesis
Country:ChinaCandidate:L YanFull Text:PDF
GTID:2143360215467785Subject:Pesticides
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Recently, more attention has been paid on products of natural plants which play an important role for impoldering substitutes of organic synthetic pesticides which are serious threat to the environment such as resistance, residue and resurgence, especialy high toxicity to vertebrates. In order to develop new ways and new natural products for controlling Ditylenchus Destructor Thorne, nematicidal acitivities of 14 species plants to Ditylenchus Destructor were studied with bioassay in vivo in present paper. Nematicidal components of Ginkgo biloba L. were also initially separated. Meanwhile, nematicidal acitivities of 15 phytochemicals were also investigated, and the results would provide some theoretical basis for the research and development of friendly nematicides. The results could be summarized as follows:1. In vitro bioassay results showed that, only nematicidal activity to Ditylenchus Destructor of Ginkgo biloba L. and Lantana camara L. with 3 different extracts are above 90% in 14 plants;And it is the first report about nematicidal activity of Ginkgo biloba L., Vitex negundo L. and Abutilon theophrasti Medic. to Ditylenchus Destructor. The results also indicated that the nematicdal components mainly existed in the strong polarity solvents extracts.2. The difference among nematicidal activities of benzene extract(BeOH-Ext.) of Ginkgo biloba L. prepared with different methods was remarkable, and the nematicidal components of Ginkgo biloba L. existed in petroleum ether extraction of BeOH-Ext.3. Toxicity assay results showed that the obvious nematicidal activities were indicated to 15 tested phytochemicals except Aloperine, Matrine, Physostigmine, Hydroquinone and Phloroglucinol against Ditylenchus Destructor. Sophoradine, Sophoramie and Sophocarpine could clobber Ditylenchus Destructor fleetly, but some nematodes clobbered may resurge for a certain time, it could be illuminated that nematicidal activities of these 3 compounds were reversible.
Keywords/Search Tags:Ginkgo biloba L., Ditylenchus Destructor, bioactivity, phytochemicals
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