Analyse Of Diversity And Evolution Of MtDNA D-loop Sequence In Five Chinese Sheep Populations

In order to study the evolution and sequence difference of Chinese sheep population mitochondrial DNA D-loop, Sequenced mtDNA D-loop of five Chinese sheep populations, the results shows, in Chinese sheep, the length of mtDNA D-loop is 1182bp。the percentage of A, T, G, C is 34.66%,28.94%,13.90%,22.50%, A+T is 63.60%, G+C is 36.40%, visibly, the content of A+T is higher than G+C。Found 101 polymorphism sits, contains 85 transition,10 transvertion and 6 insertion/deletion,the percentage of polymorphism sits is 84.16%,9.9%,5.94%。Have not found variation site in one time, 47 sits are two times variation, 1 site is found 3 times variation, the percentage of polymorphism is 0%,46.53%,0.99%,there were 53 parsimony informative site were two nucle variation,the percentage of variation site is 52.48%, not found 3 nucleotide variation parsimony informative site。Nucleotide diversity Pi is 0.03023。In 25 Chinese sheep's mtDNA D-loop sequence, there are 23 sequences have 4 motifs, the percentage is 92%, two sequence have 3 motifs, the percentage is 8%,not found sequence have 5 motifs。There are 21 haplotypes in 23 domestic sheep, can be classified in three main haplotypes: I, II and III, the percentage is 66.67%(14/21),28.57%(6/21),4.76%(1/21), the analyses of genetic distance between them show that GanSu Tan sheep is different than other 4 populations, is 0.0459~0.0573, the other four populations are stable, is 0.0246~0.0342。using Neughbor-Joining,UPGMA and maximum parsimony distance method drawn Phylogeny tree and UPGMA tree, these trees are classified into three internodes clearly, so consider that Chinese sheep populations may have three mother genesis。...