Studies On Difference And Stability Of Waterlogging-tolerance In Barley Doubled Haploid Lines (Taixing9425×Franklin)

77 barley DH lines (Taixing9425×Franklin) which were constructed by Cooperation Item between China and Australia and their parents were used as material for researching the waterlogging tolerance in barley. Waterlogging treatments were carried respectively at seedling stage (03/2005) and booting stage (12/2005). The differences of the influence of waterlogging in 9 barley plant traits (plant high(PH), spike length(SL), the top 1st internode length (TFIL), kernels of main spike(KMS),spikes per plant(SP),kernels per plant(KP), dry weight per plant(DWP), kernel weight per plant(KWP),and kilo-grain weight(KGW)), 3 young plant traits (leaf age(LA), the tiller number(TN) and the yellow-leaf number(YLN)) were studied. The rank analysis and dynamic clustering were used to study the waterlogging-tolerance and tolerance stability of the different DH lines. The results were as follows:1. Plant traits in different lines were reached significantly differences with different waterlogging treatments. Both of the two different waterlogging treatments were significantly restrained the plant traits except KGW at booting stage waterlogging treatment. It was showed that waterlogging tolerance widely influenced the plant traits, whatever the stage of treatments. There were significant genetic differences among the DH lines.2. Different waterlogging treatments made different influence on plant traits. The average percent of waterlogging influence in plant traits showed that, 1). Most coefficients of variation in plant traits were exceeded 50%, few were even exceeded 100%; 2). The waterlogging influence percent of all the plant traits were higher than 0 except KGW at booting stage and SP at seedling stage waterlogging treatment; 3). The most different influences in plant traits were SP and KGW. All the above implied that different waterlogging treatments influenced most of the plant traits, but the degree of influence were different on traits; the percentage of waterlogging influence on plant traits were different between DH lines; the waterlogging tolerance of DH lines was related with the waterlogging treatment stage and the different climate of years.3. The clustering analysis and stability analysis of waterlogging tolerance in DH population indicated that, the mid-tolerance and low-tolerance lines had better stability in different waterlogging treatments and years. Most high-tolerance lines were instability in different years and waterlogging treatments, but there were also many stabled high-tolerance lines ( TF085 and TF133 ).4. Dynamic clustering was firstly used to identify the waterlogging tolerance combining of physiologist index and plant traits indexes. 6 stabled lines were identified high-tolerance at seedling and maturing stage, including TF050,TF109,TF122,TF123,TF137 and TF150.