Cloning And Function Analysis Of TaSX In Wheat (Triticum Asetivum L.)

Salt stress is one of the major abiotic stresses in agriculture worldwide. Two dimensional electrophoresis (2-DE) and MS was used by Fengxue to analyze the proteome of the salt–tolerant mutant of wheat (RH8706-49) treated with 150mM NaCl for 48h. Total proteins of roots were extracted and separated by two-dimensional gel electrophoresis. There are 6 spots were reproducibly detected, these protein spots are selected for MS. We postulated the proteins unmatched to known proteins in the database were new proteins. The results are analyzed by Mascot and DNAStar. We find that NO.5 candidate protein spot is an unknown protein in rice, and its Nominal mass & Calculated pI value is identified with the map of gel, so we can carry it further study. We blast its cDNA on NCBI and contig ESTs that is found in wheat. A cDNA fragment including whole ORF is assembled and obtained by RT-PCR. The gene is named TaSX. The roots of RH8706-49 were treated by ABA,PEG,NaCl within 0h,1h,6h,12h,72h. The change of TaSX's expression can be detected by Real-time PCR. The binary expressing vectors, p1300-TaSX were constructed basing on the clone vector and were transferred into agrobactrium GV3101 with the control vector p1300.