The Heterosis And Combining Ability Analysis Of Kenaf And Identification Of The RAPD Markers Linked To The Male Sterility Gene

28 hybrid combinations were made from 8 kenaf inbred lines with Griffing complete design 2 in this study. The synthetic heterosis and combining ability of yield characters were analyzed.The colony heterosis——a new concept was brought forward. It is more important that we put forward a new method to evaluate the synthetic heterosis of several characters. At last a new parameter of the synthetic colony heterosis index was got. The evaluation result of the synthetic colony heterosis index was very rational and consistent with the result of combining ability analysis. These imply the synthetic colony heterosis index can roundly and impersonally estimate the real quality of the hybrid combination. The result is better than the evaluation result from single character.It avoided the unilateralism and limitation of evaluating heterosis with single character.Combining ability analysis can estimate the quality of inbred line, which can be used as the bases of making hybrid combinations. The effective value of the general combining ability is the basic of making and selecting hybrid combination. So the general combining ability must be considered in the first place in making hybrid combination, then the specific combining ability. According to the result of combining ability analysis, P1 is an excellent inbred line, its general combining ability is much higher than others in many characters, P2 and P3 is also very good. The specific combining ability of P1×P8 is notably higher than others in 4 characters.The DNA of every plant was extracted to constitute the gene pool by BSA (bulked segregant analysis). Applying the RAPD technique we analysised the two gene pool. About 800 primers were screened, and two primers were found which showed the difference between the fertile gene pool and sterile gene pool. The two primers are S393 (5'-ACCGCCTGCT-3') and SI332(5'-GGTCGGGTCA-3'), and the two bands are S393₁₅₀₀and S1332₂₃₀₀. The two special bands were found in many of the F2 sterile individuals, while not found in many of the F2 fertile individuals. This means the two loci are linked with the male sterility gene.