RAPD Study On Wild Soybean (G. Soja) And Cultivated Soybean From Different Latitude In China

DNA genetic variation of 22 accessions of wild soybean and 22 accessions of cultivated soybean, which were from different latitudes in China, was studied by RAPD technique. Forty pairs of soybean RAPD primers were synthesized according to published data, and 12 out of them had polymorphic alleles. Specific bands were amplified by primer 3 between wild soybean and cultivated soybean, which showed this RAPD marker was one of the alleles among wild soybean and cultivated soybean. The data analysis showed that: The average genetic distance of wild soybean and cultivated soybean were 0.176 and 0.150 respectively which agreed on the thesis that wild soybean had more polymorphism than that of cultivated soybean. And also it provided a molecular proof that wild soybean and cultivated soybean belonged to two species from the dendgrom at the site of genetic distance 0.300, which conformed to phenotypic results.