Establishment Of A Mouse Model Infected With Eperythrozoon Suis

The Eperythrozoonosis suis was produced by Epecythrozoon suis whichparasite on erythrocyte surface and free in blood plasma and marrow ofswine, which have symptom of piglets febrile, anemia, jaundice and originabortion. This disease always happened with other disease of swine. Itwas difficult to prevention and cure in clinic. So it took a great ofeconomic losses to raise pigs. So far, we can't enact effectivepreventive measure because the study of etiology, epidemiology,immunology of E. suis even inadequate. In order to lain the foundation ofstudy E. suis thoroughly, a suitable and stabile animla model of E. suisshould be establish.To establish a KunMing mice model associated with E. suis followingsplenectomy or injected dexamethasone(DEX) to depress their immunity.E. suis was inoculated into KunMing mice by peritoneal injection. Theidentification was being by blood smears, PCR examination and sequencing,clinical symptom and pathological change. The results indicated that eachgroup mice could be infected with E. suisby PCR examination after infected1-2d, the infection rate ofsplenectomy and injected DEX miceachieve peakafter infected 4-5d; the infection rate of mice of normal mice achievepeak after infected 7d. The homology of mice of infected E. suis andE. suis-Jilin is 100ï¼…by sequencing. The antibody titer of E. suis ofsplenectomy and injected DEX mice appear temporal step up whereafterdegrade gradually, the antibody titer of E. suis of normal mice escalateand achieve highest by 21d. In the stage of experiment, normal mice didn'tappear evident clinical symptom constantly but low immunity mice showed clinical symptom as Eperythrozoonosis suis like clothing hair backward,ademosyne, mucous membrane pallid and xanthochromia. This studyestablished the mouse model infected with E. suis successfully.