| The storage behavior of'Pink Lady'apples picked at 3 mature stages and the effects of 0.5μL·L-11-methylcyclopropene (1-MCP) on respiration, ethylene production, ACO (ACC oxidase) activity, storage quality and soluble proteins in apples with different maturity were studied during 6-months cold storage. The results were as follows.(1). Harvest maturity influenced storage quality of'Pink Lady'apples stored at 0℃, especially when the fruits were stored over 3 months. The more mature the fruits were harvested, the more loss happened in flesh firmness, titratable acids and soluble solids after long-term storage. Therefore, it was recommended that the fruit expected for a long-term storage be harvested at the late middle of October and the fruit expected for a short-term storage be harvest by the end of October.(2). 1- MCP treatment (0.5μL·L-1 at 20℃for 24 hour) significantly inhibited the respiration, ethylene (C2H4) production and ACO activity, and slowed down the decrease in the flesh firmness and titratable acids of'Pink Lady'apples during storage at 0℃. Some differences in the respiratory and ethylene peaks of fruits picked at three harvest dates were found. However there was no difference in the effects of 1-MCP on storage quality of 3-harvested fruits.(3). SDS-PAGE analysis of soluble proteins showed three new protein bands, 37.1, 18.0, 16.6kD, in untreated fruit during 6-months storage at 0℃. The appearance of these proteins were delayed in 1- MCP (0.5μL·L-1 )treated fruits. It is speculated that those new proteins are related to the apple senescence and their synthesis in apples is regulated by ethylene. |
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