The Effect Of Nitrogen Rate And Forms On Pigment Protein Complexes Of Leaf In Wheat Seedling

The experiment was carried out to study the effect of nitrogen rate and form on the pigment-protein complexs under the condition of hydroponics. The wheat which was one of widely cultivated crop was used as the material.The contents of pigment protein complexes,the absorption spectra,fluorescence emission spectra and excitation spage of chloroplast thylakoid-membrane,the content of protein and chlorophyll of wheat seedling and Hill reaction activity of different deals were analysised. The results were as follows:1. Under different level of nitrogen,the contents of pigment protein complexes of PSⅡwere decreased to a low level as the level of nitrogen decreased. That were indicated that the amounts of 24～30 kD polypeptides of LHCⅡin particular. But the nitrogen change were not impact on the pigment protein complexes of PSI.;Under different forms of nitrogen,the amounts of 31～43 KD polypeptides of LHCⅡa nd the contents of chlorophyll- protein complexes of PSⅠwere increased to a top rate at 50% NH4+-N +50% NO3--N.2. The absorption spectra,fluorescence emission spectra and excitation spage of chloroplast thylakoid-membrane (normal temperature) increased gradually as the level of nitrogen increased.3. The content of protein of wheat seedling chlorophyll reached to the tiptop at 16.86mg·L-1 of nitrogen; Under different forms of nitrogen, chlorophyll protein reached to the tiptop at 50% NH4+-N +50% NO3--N.4. the contents of chlorophyll of wheat seedling chlorophyll reached to the tiptop at 5.62mg·L-1 of nitrogen;Under different forms of nitrogen, chlorophyll protein reached to the tiptop at 50% NH4+-N +50% NO3--N.5. The Hill reaction activity decreased as the rate of NH4+-N decreased.