The Effect Of Different Additive On Oocyte Maturation,NT Embryos' Development And Oocytes' Development After Cryopreservation

Effect of addition of EGF,OCS,FBS,BFF and VE in culture medium on the in vitro maturation of bovine oocytes,then forming bovine SNT embryos by the microinjection of cytoplasm ,study the influce of nuclear and cytoplasm maturation,the results showed:1. Adding EGF to OM media raising the rate of oocytes'maturation(P<0.05) and NT embryos'cleavage(P<0.01) and blastodysts(P<0.01).2. Adiding OCS(0d) or OCS(7d) to OM,the rate of maturation in group of OCS(0d) were higher significantly than that of OCS(7d)(P<0.01);the rate of cleavage in group of OCS(0d) is higher than than of OCS(7d) (P>0.05); the rate of blastocysts in group of OCS(0d) were higher significantly than that in group of OCS(7d)(P<0.01).3. Adding OCS(0d) or FBS to OM,the rates of maturation,cleavage and blastocysts in group of OCS(0d) were all higher significantly than that of FBS(P<0.01).4. Adding 5%BFF+5%FBS or 10%BFF to OM, the rate of maturation in group of 5%BFF+5%FBS were higher significantly than that of 10%BFF(P<0.05);the rate of cleavage in group of 5%BFF+5%FBS is 84.61%,the rate of blastocysts is 14.29%.5. Adding VE or not to OM,the rates of maturation is higher than that of control(P>0.05),cleavage and blastocysts in group of VE were all higher than that of control(P<0.01).Cryopreservation oocytes that treatment in OM adding EGF,OCS and VE or not separately,then thawing oocytes,evaluation the influence of freezing oocytes that different additive in OM using the rate of oocytes'eumorphism and development capacity IVF embryo. the results showed:1. The rate of oocytes'eumorphism in control group and that adding EGF were 71.9% and 76.2% separately(P>0.05),the rate of cleavage in two groups were 63.4% and 79.7% separately(p﹤0.05),the rate of blastocysts in two groups were 15.1% and 30.3% separately.2. The rate of oocytes'eumorphism in control group and that adding OCS were 70.5% and 75.7% separately(P>0.05),the rate of cleavage in two groups were 65.2% and 81.4% separately(p﹤0.05),the rate of blastocysts in two groups were 13.3% and 26.7% separately(p﹤0.05).3. The rate of oocytes'eumorphism in control group and that adding VE were 73.9% and 79.2% separately(P>0.05),the rate of cleavage in two groups were 60.8% and 68.6% separately(p>0.05),the rate of blastocysts in two groups were 14.1% and 18% separately(p>0.05).