Introducing Vectors With Anti-waxy Gene Into The Male Sterile Line To Improve The Tasting Quality Of Rice

In recent years, with large amount of transgenic plants being commercialized, people feel uneasy about the potential risks of transgenic plants. Marker-free and acceptor promoter may raise food and environment biosafety of Genetically Modified Crops,so it becomes more and more important to abtain safety transgenic plants.In the first part of the study,the two kinds of Agrobactium tumefaciens, which respectively carried the vector pCAMBIA1301 (contains hygromycin gene and gus gene) and vector p13AWY-1, which carried aim gene (anti-waxy gene) only but without resistance marker gene (hygromycin) and 35S promoter,were mixed at the density ratio of 1:9. The T-DNAs of the two vectors pCAMBIA1301 and p13AWY-1 were introduced into 261S by the co-transformation method of which is safety transgenic technique. Using PCR analysis showed that 6 lines among 46 transgenic lines were co-transformation plants, The vector p13AWY-1 T-DNA in the rice genome was confirmed through Southern blot analysis. Then using gus gene to wash out T1 seeds that have the report gene and the marker gene. It can lay the groundwork for filtrating rice materials which containing target gene, without the marker gene and report gene.The amylase analysis shows that the amylose content of some of the transgenic rice have been lower than the wild tipe.In the second part of the study,we contrast the amylose content of four lines of trandgenic rice,of which contain one copy or two copies of anti-waxy gene. It shows that amylose content is reduced obviously in the two lines of transgenic rice.,which carried two copies of anti-waxy gene by crossing the transgenic species which were introduced one copy of anti-waxy gene.It also showed that the average amylose contents of the two hybrid rice of F1 were 8.96% and 8.23%, and they all within reach of the extension of amylose contents of soft rice ,it meaned we could cultivate new kinds of soft rice by introducing anti-waxy gene into some rice species ,and the results also told us we could obtain the highly expressed transgenic plants by crossing the transgenic species which obtained through the same transformation.In the third part of the study,the plasmid p13AWY-2 which carried two copies of aim gene(anti-waxy) was introduced into 261S by the co-transformation method too,and we have got co- transformed plants now.It will lay the foundation for rice eating quality improvement by transgenic technology. Moreover, it can make the genetically modified plants safer for consumers and the environment.In this first and third parts of the study,we used not only the safety methord but also the marker free vectors for transforming the anti-waxy gene into the male sterile line to improve the tasting quality of rice, so the transgenic rice with aim gene only will be safety and we can hope that the transgenic products will be more easily and early planted for use.