Studies On Exotic Nitric Oxide And Spermine Enhancing Salt Tolerance Of Festuca Arundinaces Schreb

Festuca arundinaces Schreb has been treated with 0.05～0.3mol/L NaCI, We measurated the physiological changes of malondialdehyde (MDA) , praline and antioxidant enzyme ,such as superoxide dismutase(SOD), peroxidase (POD)and catalase (CAT) , of Festuca arundinaces in its salt stress. It shows there have no damage to Festuca arundinaces_ in lower salt stress.The activity of antioxidant enzyme in leaves became feeble following the increasing salt concentration, but still relative stable.The activity is lower than the comparation in the concentration of NaCI beyond 0.2mol/L.The proline content changes so obviously by thick salt stressed that three times the comparation, but little change in light salt stress. The content of MDA is stable relatively. Then it shows the cell membranes of leaves are not damaged.Both 1.0mmol/L sodium nitroprusside (SNP) and 0.1mmol/L Spermine (Spm) could strengthen the salt resistance of Festuca arundinaces Schreb to salt stress. At first, the content of proline is relative rising in different salt concentration after treat with 1.0mmol/LSNP and 0.1mmol/L Spm. Proline is a nontoxic substance and one of the key regulators of Festuca arundinaces to regulate penetrability in thick salt conditions. Secondly, exotic 1.0mmol/LSNP and 0.1mmol/L Spm decrease the content of MDA in leaves of Festuca arundinaces.MDA is a symbol of extent to damaged cell membranes. Antioxidant enzyme is one of the main Mechanisms to salt tolerance.The activitives of SOD, POD and CAT in leaves are potentiating after treated with1.0mmol/LSNP and 0.1mmol/L Spm, its weakened extents in salt stress have been prevented by using SNP and Spm.The effect to CAT is obviously but not obciously to SOD and POD. CAT is the main Antioxidant enzyme to induce in order to enhance the salt resistance.The molecule structure and chemic character are more different, but there effects to Festuca arundinaces are uniform. They are all signal molecule, Antioxidant, participate in some physiological courses of anti-adversity. The effect of Spm to Festuca arundinaces is superior to SNP in light-moderate salt stress, but not as good as SNP in thick salt content stress. After t-test, itshows there is no distinct difference between their dffects to enhance the salt tolerance of Festuca arundinaces.