| At present,the wild species of the Polygonum multiflorumtm Thunb is on the edge of extinction,the cultivated one has just began,however,the use of cultivated seeds is on the original disorder bulk status,that will badly influence the improving of the yield and the stability of the quality. So it is crying for reinforcing the protection of wild sources and the research of introduce domestication,stepping the investigation and arrangement of germplasm on the juice and selecting the superior material which conforms GAP to promote the yield. There are several methods for the research of traditional Chinese drugs germplasm,the morphology marker,cytology marker that is konyotype analysis and anatomy identification came out originally,then followed by biochemical macromolecules marker that contains enzyme and protein fingerprint marker. To identify and category the germplasm,analysising the chemical constituent is also feasible. All the results of those methods are influenced by environment and limited by plants themselves.Their procedures are very complicated,cost too much labor and material,however, the more fatal shortcoming is the result is not the direct reflection of the character of the germplasm due to the shortcoming of their technology.The results of molecular marker can reflect the plant's inheritable character especially with the development and application of the DNA molecular fingerprint technology,it is possible for traditional Chinese drugs germplasm on the molecular level that is also on the real meaning.It has been widely used for resource management and identification,and other research of traditional Chinese drugs. This study assessed and collated Chongqing Polygonum multiflorumtm Thunb germplasm and search for genetic relationship and high-yield character among different morphology and area of Polygonum multiflorumtm Thunb germplasm. That is to theoretical base for seeds selection and breeding.1 Genetics analysis A new marker system SRAP was used to detect the polymorphisms of Polygonum multiflorumtm Thunb in Chongqing. Collect different shape of Polygonum multiflorumtm Thunb from major production areas.The SRAP was used to asses divergence among 16 populations.The data were analyzed using unweighted pairgroup method, based on arithmetic averages(UPGMA) bootstrap analysis.Cluster analyses were performed by using DPSv3.01 software,the alkaloid was extracted from P.ternate with chlorolform.104 combinations generated 250 polymorphic bands,the cluster analysis indicated 16 materials could be distinguished into two main groups and one special type,Nei&Li similarity coefficient ranged from0.23～0.99,and the average distance is 0.44.The most close two materials are numberl&2,on the contrary,the most further two materials are number 10 and 16 separatly from Shizhu and Beibei.The cluster analysis shows that affinity relationship conform their distribution areas, coincidenting with the study of WangLing-hui.But we can't differ them by their areas absoultly,there exist differentiation yet.In the stuff of Shizhu 10,11 and 13 are very close,but 9 and 12 both have theirs independence,that maybe result from the effect of circumstance and the long time evolution.In addition,polymorphisms of Polygonum multiflorumtm Thunb is self-pollinated plant,there is lower differetntintion in theory,but the author choose a very narrow area in this study,in spite of that, the results of the study showed a potential application of SRAP fingerprinting for the identification of Polygonum multiflorumtm Thunb.2 Physiologic index analysis The author mensurated the leaf area,chlorophyll content,thickness, morphologic observation,included angle and the root system activity of 17 Polygonum multiflorumtm Thunb materials respectively,the result indicated that the 17 materials' physiologic index performance is most prominent,the material 12 is a special stuff shown from the dendrogram,and it' morphology & physiologic index performance looks exceptional. The most distinctive material in genetic analysis is number 9,but it' s physiologic index is very common.3 Comprehensive study The adjacent materials of genetic distance shows very correlative in physiologic index,such as number l&2,number 10&11,number 5&12,and so on.But there is also exception,such as numberl5&16 differs largely in the leaf area, chlorophyll content, and the root system activity.Screened out some excellent materials,numberl,10 and 17,we can take them as releasing variety;number 9 and 12 could be conserced as germplasm resources.The special material in Genetics analysis are not always exclent in production,so in my opinion ,when we choose the good breed for out production,we just search them from the existing resource on hand,but we should try our best to maintain the variety puritify.If we want to study it in-depth,we had better sort the resources and identify them firstly by this way,and then we can protect diversity natural resources. |
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